{"doi":"10.1002/hed.26501","title":"Association of human papillomavirus integration with better patient outcomes in oropharyngeal squamous cell carcinoma","abstract":"BACKGROUND: The molecular drivers of human papillomavirus-related head and neck squamous cell carcinoma (HPV + HNSCC) are not entirely understood. This study evaluated the relationship between HPV integration, expression of E6/E7, and patient outcomes in p16+ HNSCCs. METHODS: HPV type was determined by HPV PCR-MassArray, and integration was called using detection of integrated papillomavirus sequences polymerase chain reaction (PCR). We investigated whether fusion transcripts were produced by reverse transcriptase polymerase chain reaction (RT-PCR). E6/E7 expression was assessed by quantitative RT-PCR. We assessed if there was a relationship between integration and E6/E7 expression, clinical variables, or patient outcomes. RESULTS: Most samples demonstrated HPV integration, which sometimes resulted in a fusion transcript. HPV integration was positively correlated with age at diagnosis and E6/E7 expression. There was a significant difference in survival between patients with vs without integration. CONCLUSIONS: Contrary to previous reports, HPV integration was associated with improved patient survival. Therefore, HPV integration may act as a molecular marker of good prognosis.","journal":"Head & Neck","year":2020,"id":101843,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":27,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9685,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":498593,"name":"Hana Sinha","orcid":null,"position":1,"is_corresponding":false},{"id":497929,"name":"Collin Brummel","orcid":"0000-0001-7198-8560","position":2,"is_corresponding":false},{"id":498594,"name":"Christine M. Goudsmit","orcid":null,"position":3,"is_corresponding":false},{"id":497930,"name":"Timothy J. Geddes","orcid":"0000-0002-1684-6428","position":4,"is_corresponding":false},{"id":108246,"name":"George D. Wilson","orcid":"0000-0003-3756-1701","position":5,"is_corresponding":false},{"id":498595,"name":"Jan Åkervall","orcid":null,"position":6,"is_corresponding":false},{"id":376564,"name":"J. Chad Brenner","orcid":"0000-0003-3238-1111","position":7,"is_corresponding":false},{"id":497931,"name":"Heather M. Walline","orcid":"0000-0003-3790-5738","position":8,"is_corresponding":false},{"id":13324,"name":"Thomas E. Carey","orcid":"0000-0002-5202-7518","position":9,"is_corresponding":false},{"id":497928,"name":"Lisa Pinatti","orcid":"0000-0002-4864-5698","position":0,"is_corresponding":true}],"reference_count":51,"raw_metadata":null,"created_at":"2026-07-18T22:40:22.678835Z","pmid":"33073473","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}