{"doi":"10.1002/gcc.21963","title":"Combined analysis of <i>TMPRSS2–ERG</i> and <i>TERT</i> for improved prognosis of biochemical recurrence in prostate cancer","abstract":"<jats:title>Abstract</jats:title><jats:p>Prostate cancer (PCa) is a heterogeneous disease with diverse clinical outcomes. <jats:italic>TMPRSS2–ERG</jats:italic> is the most common gene fusion in PCa, whereas activation of telomerase is a common feature of various malignancies. The aim of our study was to explore the combined utility of these and some other biomarkers in predicting biochemical recurrence after radical prostatectomy. Prostate specimens and urine sediments from 179 previously untreated patients with pT2‐pT3 stage PCa were analyzed for expression of telomerase (<jats:italic>TERT</jats:italic> and <jats:italic>TR</jats:italic>) and the <jats:italic>TMPRSS2–ERG</jats:italic> fusion gene by means of reverse transcription PCR. Real‐time PCR was used for quantification of <jats:italic>ERG</jats:italic> and <jats:italic>SPINK1</jats:italic> expression. In total, 74% (117/158) of the prostate adenocarcinomas were positive for the <jats:italic>TMPRSS2–ERG</jats:italic> and/or <jats:italic>TERT</jats:italic> expression. Noninvasively, these transcripts were identified in 31% (19/61) of catheterized urine specimens. Significantly higher expression of <jats:italic>ERG</jats:italic> was detected in <jats:italic>TMPRSS2–ERG‐</jats:italic>positive tumors (<jats:italic>P</jats:italic> &lt; 0.0001), whereas more intense expression of <jats:italic>SPINK1</jats:italic> was characteristic for the <jats:italic>TMPRSS2–ERG‐</jats:italic>negative tumors (<jats:italic>P</jats:italic> = 0.003). <jats:italic>TERT‐</jats:italic>positive cases also had elevated levels of <jats:italic>ERG</jats:italic> (<jats:italic>P</jats:italic> = 0.016), suggesting a possible link between aberrant expression of <jats:italic>ERG</jats:italic> and reactivation of <jats:italic>TERT</jats:italic> in prostate tumors. The cases negative for both transcripts, <jats:italic>TMPRSS2–ERG</jats:italic> and <jats:italic>TERT,</jats:italic> rarely recurred (<jats:italic>P</jats:italic> = 0.014) and showed significantly longer biochemical recurrence‐free period (<jats:italic>P</jats:italic> = 0.022) as compared to the <jats:italic>TMPRSS2–ERG</jats:italic> and/or <jats:italic>TERT‐</jats:italic>positive cases. The results of our study suggest that combined analysis of <jats:italic>TMPRSS2–ERG</jats:italic> and <jats:italic>TERT</jats:italic> expression can be a valuable tool for early prediction of biochemical recurrence of PCa after radical prostatectomy. © 2012 Wiley Periodicals, Inc.</jats:p>","journal":"Genes, Chromosomes and Cancer","year":2012,"id":619702,"datarank":0.47670807455219194,"base_score":3.1780538303479458,"endowment":3.1780538303479458,"self_citation_contribution":0.47670807455219194,"citation_network_contribution":0.0,"self_endowment_contribution":0.47670807455219194,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":23,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1235707,"name":"Sonata Jarmalaite","orcid":null,"position":1,"is_corresponding":false},{"id":1599426,"name":"Donatas Petroska","orcid":null,"position":2,"is_corresponding":false},{"id":1599427,"name":"Darius Dasevicius","orcid":null,"position":3,"is_corresponding":false},{"id":1599428,"name":"Arvydas Laurinavicius","orcid":null,"position":4,"is_corresponding":false},{"id":1599429,"name":"Feliksas Jankevicius","orcid":null,"position":5,"is_corresponding":false},{"id":1599430,"name":"Juozas R. Lazutka","orcid":null,"position":6,"is_corresponding":false},{"id":1599425,"name":"Rasa Sabaliauskaite","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Combined analysis of <i>TMPRSS2–ERG</i> and <i>TERT</i> for improved prognosis of biochemical recurrence in prostate cancer","abstract":"<jats:title>Abstract</jats:title><jats:p>Prostate cancer (PCa) is a heterogeneous disease with diverse clinical outcomes. <jats:italic>TMPRSS2–ERG</jats:italic> is the most common gene fusion in PCa, whereas activation of telomerase is a common feature of various malignancies. The aim of our study was to explore the combined utility of these and some other biomarkers in predicting biochemical recurrence after radical prostatectomy. Prostate specimens and urine sediments from 179 previously untreated patients with pT2‐pT3 stage PCa were analyzed for expression of telomerase (<jats:italic>TERT</jats:italic> and <jats:italic>TR</jats:italic>) and the <jats:italic>TMPRSS2–ERG</jats:italic> fusion gene by means of reverse transcription PCR. Real‐time PCR was used for quantification of <jats:italic>ERG</jats:italic> and <jats:italic>SPINK1</jats:italic> expression. In total, 74% (117/158) of the prostate adenocarcinomas were positive for the <jats:italic>TMPRSS2–ERG</jats:italic> and/or <jats:italic>TERT</jats:italic> expression. Noninvasively, these transcripts were identified in 31% (19/61) of catheterized urine specimens. Significantly higher expression of <jats:italic>ERG</jats:italic> was detected in <jats:italic>TMPRSS2–ERG‐</jats:italic>positive tumors (<jats:italic>P</jats:italic> &lt; 0.0001), whereas more intense expression of <jats:italic>SPINK1</jats:italic> was characteristic for the <jats:italic>TMPRSS2–ERG‐</jats:italic>negative tumors (<jats:italic>P</jats:italic> = 0.003). <jats:italic>TERT‐</jats:italic>positive cases also had elevated levels of <jats:italic>ERG</jats:italic> (<jats:italic>P</jats:italic> = 0.016), suggesting a possible link between aberrant expression of <jats:italic>ERG</jats:italic> and reactivation of <jats:italic>TERT</jats:italic> in prostate tumors. The cases negative for both transcripts, <jats:italic>TMPRSS2–ERG</jats:italic> and <jats:italic>TERT,</jats:italic> rarely recurred (<jats:italic>P</jats:italic> = 0.014) and showed significantly longer biochemical recurrence‐free period (<jats:italic>P</jats:italic> = 0.022) as compared to the <jats:italic>TMPRSS2–ERG</jats:italic> and/or <jats:italic>TERT‐</jats:italic>positive cases. The results of our study suggest that combined analysis of <jats:italic>TMPRSS2–ERG</jats:italic> and <jats:italic>TERT</jats:italic> expression can be a valuable tool for early prediction of biochemical recurrence of PCa after radical prostatectomy. © 2012 Wiley Periodicals, Inc.</jats:p>","is_dataset_classified":null,"base_score":3.1780538303479458,"endowment":3.1780538303479458,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"22505341","pmcid":null,"openalex_id":"https://openalex.org/W2169428763","authors":[],"funders":[],"total_grants":0,"fwci":2.6218,"citation_percentile":0.90390644,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":3},{"year":2014,"count":3},{"year":2015,"count":4},{"year":2016,"count":2},{"year":2017,"count":2},{"year":2020,"count":1},{"year":2021,"count":3},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2024,"count":1}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fgcc.21963","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/gcc.21963","host_type":"publisher"},{"url":"https://doi.org/10.1002/gcc.21963","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/22505341","host_type":"repository"}],"fields_of_study":["Prostate Cancer Treatment and Research","Cancer, Lipids, and Metabolism","Ubiquitin and proteasome pathways","Biomarkers, Tumor","Carrier Proteins","Humans","Kaplan-Meier Estimate","Male","Middle Aged","Neoplasm Recurrence, Local","Oncogene Proteins, Fusion","Prognosis","Prostatic Neoplasms","Reverse Transcriptase Polymerase Chain Reaction","Statistics, Nonparametric","Telomerase","Trans-Activators","Transcriptional Regulator ERG","Trypsin Inhibitor, Kazal Pancreatic"],"mesh_terms":["Transcriptional Regulator ERG","Carrier Proteins","Humans","Male","Middle Aged","Neoplasm Recurrence, Local","Prognosis","Prostatic Neoplasms","Trypsin Inhibitor, Kazal Pancreatic","Biomarkers, Tumor","Oncogene Proteins, Fusion","Trans-Activators","Statistics, Nonparametric","Telomerase","Reverse Transcriptase Polymerase Chain Reaction","Kaplan-Meier Estimate"],"keywords":["TMPRSS2","Erg","Prostate cancer","Prostate","Fusion gene","Prostatectomy","Medicine","Cancer research","PCA3","Biochemical recurrence","Cancer","Internal medicine","Biology","Gene","Disease","Ophthalmology","Genetics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T08:09:27.470869Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}