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I. Isoelectric focusing and polyacrylamide gel electrophoresis using PhastSystem™","abstract":"<jats:title>Abstract</jats:title><jats:p>PhastSystem, an integrated system for horizontal electrophoresis and isoelectric focusing in small gels, including automated staining and destaining, is described. Buffers for electrophoresis are supplied to the gel from buffer strips made of agarose. The separation bed is cooled by Peltier elements. All conditions of significance to the results, both during separation and development, are controlled by a microprocessor. For separation, nine programs are available, each with 9 steps; for developement, there are nine programs with 20 steps each. In this paper, we also report results using PhastSystem for purity checking and characterization of monoclonal antibodies after affinity chromatography and also for determining their digestion rate with papain.</jats:p>","is_dataset_classified":null,"base_score":4.430816798843313,"endowment":4.430816798843313,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"2466643","pmcid":null,"openalex_id":"https://openalex.org/W2083984780","authors":[],"funders":[],"total_grants":0,"fwci":9.5663,"citation_percentile":0.99307822,"influential_citations":0,"citation_trend":[{"year":2012,"count":2},{"year":2013,"count":1},{"year":2015,"count":1},{"year":2018,"count":1},{"year":2021,"count":1}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Felps.1150090104","host_type":"publisher"},{"url":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/pdf/10.1002/elps.1150090104","host_type":"publisher"},{"url":"https://doi.org/10.1002/elps.1150090104","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/2466643","host_type":"repository"}],"fields_of_study":["Advanced Proteomics Techniques and Applications","Protein purification and stability","Genetics, Bioinformatics, and Biomedical Research","Buffers","Electrodes","Electrophoresis, Polyacrylamide Gel","Gels","Isoelectric Focusing","Staining and Labeling","Temperature"],"mesh_terms":["Buffers","Electrodes","Electrophoresis, Polyacrylamide Gel","Gels","Isoelectric Focusing","Staining and Labeling","Temperature"],"keywords":["Isoelectric focusing","Chromatography","Electrophoresis","Gel electrophoresis of proteins","Polyacrylamide gel electrophoresis","Two-dimensional gel electrophoresis","Pulsed-field gel electrophoresis","Color marker","Gel electrophoresis","Chemistry","Biochemistry","Proteomics"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T04:56:22.141022Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}