{"doi":"10.1002/eji.200425322","title":"Cross-presentation of phage particle antigen in MHC class II and endoplasmic reticulum marker-positive compartments","abstract":null,"journal":"European Journal of Immunology","year":2005,"id":639416,"datarank":1.5790248158616693,"base_score":3.332204510175204,"endowment":3.332204510175204,"self_citation_contribution":0.49983067652628066,"citation_network_contribution":1.0791941393353888,"self_endowment_contribution":0.49983067652628066,"citer_contribution":1.0791941393353888,"corpus_percentile":null,"corpus_rank":null,"citation_count":27,"citer_count":27,"citers_with_citation_signal":24,"citers_with_endowment":24,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1118039,"name":"Yuzhang Wu","orcid":"0000-0002-5948-1596","position":1,"is_corresponding":false},{"id":1661262,"name":"Jingran Zhou","orcid":null,"position":2,"is_corresponding":false},{"id":602053,"name":"Liyun Zou","orcid":"0000-0001-5449-8869","position":3,"is_corresponding":false},{"id":1661263,"name":"Yunfei Liang","orcid":null,"position":4,"is_corresponding":false},{"id":1291528,"name":"Jianping Zhao","orcid":"0000-0002-0257-0133","position":5,"is_corresponding":false},{"id":1661264,"name":"Zhengcai Jia","orcid":null,"position":6,"is_corresponding":false},{"id":1638128,"name":"Jan Engberg","orcid":null,"position":7,"is_corresponding":false},{"id":1365520,"name":"Jiang Bian","orcid":"0000-0002-9472-600X","position":8,"is_corresponding":false},{"id":407589,"name":"Wei Zhou","orcid":"0000-0001-7540-2459","position":9,"is_corresponding":false},{"id":548311,"name":"Ying Wan","orcid":"0000-0001-5909-2892","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Cross-presentation of phage particle antigen in MHC class II and endoplasmic reticulum marker-positive compartments","abstract":"It has been shown that exogenous antigens can access the MHC class I pathway of professional antigen-processing cells. However, details as to how the MHC class I-peptide complex forms in the presentation pathway are still poorly understood. Here we used MHC class I-peptide-specific antibodies to investigate the formation and intracellular location of class I-peptide complexes in macrophages. We observed that the formation of class I-peptide complexes occurs within a few hours and lasts for another few hours on the cell surface of macrophages following loading with filamentous phage particles. The class I-peptide complexes in the process were co-localized with MHC class II molecules and endocytic system markers. Moreover, endosomal compartments containing class I-peptide complexes were found within intracellular organelles stained by DiOC6 and calnexin. In addition, the cross-presentation of phage particles was transporter associated with antigen processing (TAP)-dependent and sensitive to proteasome inhibitors and NH(4)Cl. These data suggest that endocytosed phage particles may be processed and cross-presented in organelles positive for phagosome and endoplasmic reticulum (ER) markers via a classical ER MHC class I loading mechanism.","is_dataset_classified":null,"base_score":3.332204510175204,"endowment":3.332204510175204,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"15940671","pmcid":null,"openalex_id":"https://openalex.org/W1999204382","authors":[],"funders":[],"total_grants":0,"fwci":0.6966,"citation_percentile":0.67051164,"influential_citations":0,"citation_trend":[{"year":2012,"count":2},{"year":2013,"count":1},{"year":2015,"count":3},{"year":2016,"count":2},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2019,"count":1},{"year":2021,"count":1},{"year":2023,"count":1},{"year":2024,"count":1},{"year":2025,"count":1},{"year":2026,"count":2}],"oa_status":"closed","license":"http://doi.wiley.com/10.1002/tdm_license_1.1","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Feji.200425322","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/full/10.1002/eji.200425322","host_type":"publisher"},{"url":"https://doi.org/10.1002/eji.200425322","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/15940671","host_type":"repository"}],"fields_of_study":["Immunotherapy and Immune Responses","T-cell and B-cell Immunology","Immune Cell Function and Interaction"],"mesh_terms":["Ammonium Chloride","Animals","Histocompatibility Antigens Class II","Antigens, Viral","Carrier Proteins","Endoplasmic Reticulum","Hydrogen-Ion Concentration","Kinetics","Lipoproteins","Mice, Inbred C3H","Mice, Inbred C57BL","Phagocytosis","Virion","Histocompatibility Antigens Class I","Biomarkers","Trans-Activators","Inovirus","Mice, Knockout","Transport Vesicles","Cross-Priming","Mice","Proteasome Inhibitors"],"keywords":["Transporter associated with antigen processing","MHC class I","Antigen processing","Biology","Endosome","Endoplasmic reticulum","Antigen presentation","CD74","Cell biology","Cross-presentation","Antigen","Calnexin","Major histocompatibility complex","MHC restriction","MHC class II","Phagosome","Intracellular","Calreticulin","T cell","Immune system","Immunology"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-07T00:18:30.218866Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}