{"doi":"10.1002/eji.1830221204","title":"Polyreactive autoantibodies to negatively charged epitopes following <i>Trypanosoma cruzi</i> infection","abstract":"<jats:title>Abstract</jats:title><jats:p>During the course of many human autoimmune diseases, antibodies which recognize negatively charged epitopes on self antigens are detected. <jats:italic>Trypanosoma cruzi</jats:italic>, an intracellular protozoan parasite capable of infecting a wide variety of vertebrates, is the cause of Chagas disease in humans. Infection with the parasite frequently results in autoimmune and inflammatory pathology. We report here on an affinity‐purified population of antibodies that bind to a broad class of antigens that contain runs of acidic amino acids, including tubulin. Although these antibodies can be isolated from both uninfected and <jats:italic>T. cruzi</jats:italic> chronically infected C3H/He mice, the antibodies from the normal mice (the natural autoantibodies) bind to tubulin poorly at physiological pH, whereas the antibodies isolated from the infected animals bind well at physiological pH. We propose that similar processes may occur in humans following other infections accounting for the detection of antibodies to negatively charged epitopes in a variety of autoimmune diseases.</jats:p>","journal":"European Journal of Immunology","year":1992,"id":679219,"datarank":0.31191623125197543,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.0,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":660522,"name":"Maria Kahn","orcid":"0000-0001-6032-9893","position":1,"is_corresponding":false},{"id":1774654,"name":"Harvey Eisen","orcid":null,"position":2,"is_corresponding":false},{"id":1774653,"name":"Stuart Kahn","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Polyreactive autoantibodies to negatively charged epitopes following <i>Trypanosoma cruzi</i> infection","abstract":"<jats:title>Abstract</jats:title><jats:p>During the course of many human autoimmune diseases, antibodies which recognize negatively charged epitopes on self antigens are detected. <jats:italic>Trypanosoma cruzi</jats:italic>, an intracellular protozoan parasite capable of infecting a wide variety of vertebrates, is the cause of Chagas disease in humans. Infection with the parasite frequently results in autoimmune and inflammatory pathology. We report here on an affinity‐purified population of antibodies that bind to a broad class of antigens that contain runs of acidic amino acids, including tubulin. Although these antibodies can be isolated from both uninfected and <jats:italic>T. cruzi</jats:italic> chronically infected C3H/He mice, the antibodies from the normal mice (the natural autoantibodies) bind to tubulin poorly at physiological pH, whereas the antibodies isolated from the infected animals bind well at physiological pH. We propose that similar processes may occur in humans following other infections accounting for the detection of antibodies to negatively charged epitopes in a variety of autoimmune diseases.</jats:p>","is_dataset_classified":null,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"1280220","pmcid":null,"openalex_id":"https://openalex.org/W1983059021","authors":[],"funders":[{"funder_name":"NICHD NIH HHS","grant_id":"HD-22297","title":null},{"funder_name":"NICHD NIH HHS","grant_id":"HD-28586","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"AI-27803","title":null}],"total_grants":3,"fwci":0.6174,"citation_percentile":0.64670401,"influential_citations":0,"citation_trend":[{"year":2014,"count":1},{"year":2018,"count":1},{"year":2021,"count":1},{"year":2026,"count":1}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Feji.1830221204","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/eji.1830221204","host_type":"publisher"},{"url":"https://doi.org/10.1002/eji.1830221204","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/1280220","host_type":"repository"}],"fields_of_study":["Trypanosoma species research and implications","Toxin Mechanisms and Immunotoxins","Monoclonal and Polyclonal Antibodies Research","3T3 Cells","Amino Acid Sequence","Animals","Autoantibodies","Base Sequence","Chagas Disease","Epitopes","Female","Hydrogen-Ion Concentration","Mice","Mice, Inbred C3H","Molecular Sequence Data","Rabbits","Tubulin"],"mesh_terms":["Amino Acid Sequence","Animals","Epitopes","Autoantibodies","Base Sequence","Female","Hydrogen-Ion Concentration","Mice, Inbred C3H","Molecular Sequence Data","Rabbits","Chagas Disease","Tubulin","3T3 Cells","Mice"],"keywords":["Trypanosoma cruzi","Epitope","Biology","Chagas disease","Antibody","Autoantibody","Antigen","Immunology","Virology","Intracellular parasite","Parasite hosting","Population","Intracellular","Immune system","Biochemistry","Medicine"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T12:32:59.336151Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}