{"doi":"10.1002/cyto.a.24197","title":"Immunophenotyping of Rhesus <scp>CMV</scp>‐Specific <scp>CD8</scp> T‐Cell Populations","abstract":"A vaccine to ameliorate cytomegalovirus (CMV)-related pathogenicity in transplantation patients is considered a top priority. A therapeutic vaccine must include components that elicit both neutralizing antibodies, and highly effective CD8 T-cell responses. The most important translational model of vaccine development is the captive-bred rhesus macaque (Macaca mulatta) of Indian origin. There is a dearth of information on rhesus cytomegalovirus (rhCMV)-specific CD8 T cells due to the absence of well-defined CD8 T-cell epitopes presented by classical MHC-I molecules. In the current study, we defined two CD8 T-cell epitopes restricted by high-frequency Mamu alleles: the Mamu-A1*002:01 restricted VY9 (VTTLGMALY aa291-299) epitope of protein IE-1, and the Mamu-A1*008:01 restricted NP8 (NPTDRPIP aa96-103) epitope of protein phosphoprotein 65-2. We developed tetramers and determined the level, phenotype, and functional capability of the two epitope-specific T-cell populations in circulation and various tissues. We demonstrated the value of these tetramers for in situ tetramer staining. Here, we first provided critical reagents and established a flow cytometric staining strategy to study rhCMV-specific T-cell responses in up to 40% of captive-bred rhesus macaques. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals LLC on behalf of International Society for Advancement of Cytometry.","journal":"Cytometry Part A","year":2020,"id":74923,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9565,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":391897,"name":"Logan J. Vosler","orcid":null,"position":1,"is_corresponding":false},{"id":362825,"name":"Kim L. Weisgrau","orcid":null,"position":2,"is_corresponding":false},{"id":391898,"name":"Jessica Furlott","orcid":null,"position":3,"is_corresponding":false},{"id":332158,"name":"Andrea M. Weiler","orcid":"0000-0002-7130-8235","position":4,"is_corresponding":false},{"id":391274,"name":"Hadia M. Abdelaal","orcid":"0000-0002-1830-864X","position":5,"is_corresponding":false},{"id":381205,"name":"David T. Evans","orcid":"0000-0003-2498-6292","position":6,"is_corresponding":false},{"id":279513,"name":"David I. Watkins","orcid":"0000-0001-6582-3305","position":7,"is_corresponding":false},{"id":391275,"name":"Tetsuro Matano","orcid":"0000-0003-3096-6749","position":8,"is_corresponding":false},{"id":344047,"name":"Pamela J. Skinner","orcid":"0000-0003-3388-1687","position":9,"is_corresponding":false},{"id":332162,"name":"Thomas C. Friedrich","orcid":"0000-0001-9831-6895","position":10,"is_corresponding":false},{"id":286693,"name":"Eva G. Rakasz","orcid":"0000-0001-8197-5309","position":11,"is_corresponding":false},{"id":391896,"name":"Nicholas Pomplun","orcid":null,"position":0,"is_corresponding":true}],"reference_count":47,"raw_metadata":null,"created_at":"2026-07-18T21:46:11.663457Z","pmid":"32713108","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}