{"doi":"10.1002/cpz1.710","title":"Preparation of DNA and RNA Fragments Containing Guanine <i>N</i><sup>2</sup>‐Thioalkyl Tethers","abstract":"Abstract This article describes procedures for preparation of deoxyguanosine and guanosine derivatives in which the guanine N 2 contains a thiopropyl tether, protected as a tert ‐butyl disulfide. After incorporation into a DNA or RNA fragment, this tether allows site‐specific cross‐linking to a thiol of a protein or another nucleic acid. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1 : Preparation of diisopropyl‐1‐( tert ‐butylthio)‐1,2‐hydrazinedicarboxylate ( 4 ) Basic Protocol 2 : Preparation of the 2′‐deoxyguanosine N 2 ‐propyl‐ tert ‐butyl disulfide phosphoramidite ( 12 ) Basic Protocol 3 : Preparation of the guanosine N 2 ‐propyl‐ tert ‐butyl disulfide phosphoramidite ( 20 ) Basic Protocol 4 : Preparation of DNA fragments containing N 2 ‐propyl‐ tert ‐butyl disulfide guanine Alternate Protocol : Preparation of RNA fragments containing N 2 ‐propyl‐ tert ‐butyl disulfide guanine Basic Protocol 5 : Conversion of N 2 ‐propyl‐ tert ‐butyl disulfide to the free thiol, disulfide 5‐thio‐2‐nitrobenzoic acid disulfide, or ethylamine disulfide","journal":"Current Protocols","year":2023,"id":403861,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9522,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":894200,"name":"Gang Wang","orcid":"0000-0003-4882-5389","position":1,"is_corresponding":false},{"id":982895,"name":"Barbara L. Gaffney","orcid":null,"position":2,"is_corresponding":false},{"id":982405,"name":"Roger A. Jones","orcid":"0000-0002-6835-6615","position":3,"is_corresponding":false},{"id":1181446,"name":"Xiaorong Hou","orcid":"0000-0002-4903-9489","position":0,"is_corresponding":true}],"reference_count":33,"raw_metadata":null,"created_at":"2026-07-19T01:20:40.264741Z","pmid":"36943108","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}