{"doi":"10.1002/cpz1.70043","title":"Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells","abstract":"Proximity ligation assays (PLAs) use specific antibodies to detect endogenous protein-protein interactions. PLAs are a highly useful biochemical technique that allow two proteins within proximity to be visualized with fluorescent probes amplified by PCR. While this technique has gained prominence, the use of a PLA in mouse skeletal muscle (SkM) is novel. In this article, we discuss how the PLA method can be used in SkM to study the protein-protein interactions within mitochondria-endoplasmic reticulum contact sites (MERCs). © 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol: Proximity ligation assay for skeletal muscle tissue and myoblast for MERC proteins.","journal":"Current Protocols","year":2025,"id":561580,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9552,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":272272,"name":"Han Le","orcid":"0000-0002-4515-261X","position":1,"is_corresponding":false},{"id":1247540,"name":"Chanel Harris","orcid":null,"position":2,"is_corresponding":false},{"id":1169966,"name":"Ashton Oliver","orcid":"0009-0001-9874-2621","position":3,"is_corresponding":false},{"id":1463924,"name":"Andy Barillas","orcid":null,"position":4,"is_corresponding":false},{"id":1447238,"name":"Johnathan Moore","orcid":"0000-0002-3207-5360","position":5,"is_corresponding":false},{"id":1447638,"name":"Desiree Nguyen","orcid":null,"position":6,"is_corresponding":false},{"id":1463925,"name":"Izabella Marie Rabago","orcid":null,"position":7,"is_corresponding":false},{"id":97862,"name":"Blanca Rodríguez","orcid":"0000-0001-6361-3339","position":8,"is_corresponding":false},{"id":437193,"name":"Dominique C. Stephens","orcid":null,"position":9,"is_corresponding":false},{"id":338900,"name":"Heather K. Beasley","orcid":"0000-0002-4636-2889","position":10,"is_corresponding":false},{"id":618494,"name":"Edgar Garza-López","orcid":"0000-0003-0243-0816","position":11,"is_corresponding":false},{"id":618493,"name":"Kit Neikirk","orcid":"0000-0003-0994-0333","position":12,"is_corresponding":false},{"id":397855,"name":"Margaret Mungai","orcid":"0000-0003-3292-1552","position":13,"is_corresponding":false},{"id":822215,"name":"Larry Vang","orcid":"0000-0001-9098-3650","position":14,"is_corresponding":false},{"id":329475,"name":"Zer Vue","orcid":"0000-0002-3502-8906","position":15,"is_corresponding":false},{"id":874365,"name":"Neng Vue","orcid":null,"position":16,"is_corresponding":false},{"id":655224,"name":"Andrea G. Marshall","orcid":"0000-0001-9997-4478","position":17,"is_corresponding":false},{"id":1094776,"name":"Kyrin Turner","orcid":null,"position":18,"is_corresponding":false},{"id":619679,"name":"Jianqiang Shao","orcid":null,"position":19,"is_corresponding":false},{"id":307534,"name":"Sandra Murray","orcid":"0000-0002-8267-9138","position":20,"is_corresponding":false},{"id":351524,"name":"Jennifer A. Gaddy","orcid":"0000-0002-2192-4224","position":21,"is_corresponding":false},{"id":401488,"name":"Celestine N. Wanjalla","orcid":"0000-0001-9159-5414","position":22,"is_corresponding":false},{"id":720867,"name":"Jamaine Davis","orcid":"0000-0002-5078-1306","position":23,"is_corresponding":false},{"id":351523,"name":"Steven M. Damo","orcid":"0000-0002-1483-7712","position":24,"is_corresponding":false},{"id":297596,"name":"Antentor Hinton","orcid":"0000-0002-7730-952X","position":25,"is_corresponding":false},{"id":873625,"name":"Amber Crabtree","orcid":"0000-0003-1156-1658","position":0,"is_corresponding":true}],"reference_count":18,"raw_metadata":null,"created_at":"2026-07-19T02:55:58.259104Z","pmid":"39906960","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}