{"doi":"10.1002/cpmc.102","title":"<i>Giardia lamblia</i>: Laboratory Maintenance, Lifecycle Induction, and Infection of Murine Models","abstract":"<jats:title>Abstract</jats:title><jats:p><jats:italic>Giardia lamblia</jats:italic> is a protozoan parasite that is found ubiquitously throughout the world and is a major contributor to diarrheal disease. <jats:italic>Giardia</jats:italic> exhibits a biphasic lifestyle existing as either a dormant cyst or a vegetative trophozoite. Infections are typically initiated through the consumption of cyst‐contaminated water or food. <jats:italic>Giardia</jats:italic> was first axenized in the 1970s and can be readily maintained in a laboratory setting. Additionally, <jats:italic>Giardia</jats:italic> is one of the few protozoans that can be induced to complete its complete lifecycle using laboratory methods. In this article, we outline protocols to maintain <jats:italic>Giardia</jats:italic> and induce passage through its lifecycle. We also provide protocols for infecting and quantifying parasites in an animal infection model. © 2020 Wiley Periodicals LLC.</jats:p><jats:p><jats:bold>Basic Protocol 1</jats:bold>: In vitro maintenance and growth of <jats:italic>Giardia</jats:italic> trophozoites</jats:p><jats:p><jats:bold>Basic Protocol 2</jats:bold>: In vitro encystation of <jats:italic>Giardia</jats:italic> cysts</jats:p><jats:p><jats:bold>Basic Protocol 3</jats:bold>: In vivo infections using <jats:italic>Giardia</jats:italic> trophozoites</jats:p>","journal":"Current Protocols in Microbiology","year":2020,"id":599907,"datarank":0.515098080672772,"base_score":3.4339872044851463,"endowment":3.4339872044851463,"self_citation_contribution":0.515098080672772,"citation_network_contribution":0.0,"self_endowment_contribution":0.515098080672772,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":30,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1367665,"name":"Danielle Shapiro","orcid":null,"position":1,"is_corresponding":false},{"id":414035,"name":"Steven M. 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Additionally, <jats:italic>Giardia</jats:italic> is one of the few protozoans that can be induced to complete its complete lifecycle using laboratory methods. In this article, we outline protocols to maintain <jats:italic>Giardia</jats:italic> and induce passage through its lifecycle. We also provide protocols for infecting and quantifying parasites in an animal infection model. © 2020 Wiley Periodicals LLC.</jats:p><jats:p><jats:bold>Basic Protocol 1</jats:bold>: In vitro maintenance and growth of <jats:italic>Giardia</jats:italic> trophozoites</jats:p><jats:p><jats:bold>Basic Protocol 2</jats:bold>: In vitro encystation of <jats:italic>Giardia</jats:italic> cysts</jats:p><jats:p><jats:bold>Basic Protocol 3</jats:bold>: In vivo infections using <jats:italic>Giardia</jats:italic> trophozoites</jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"32515871","pmcid":"PMC7339241","openalex_id":null,"authors":[],"funders":[{"funder_name":"National Science Foundation","grant_id":"AI‐094492","title":null},{"funder_name":"National Science Foundation","grant_id":"AI‐109591","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R15 AI094492","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R15 AI109591","title":null},{"funder_name":"National Institutes of Health","grant_id":"1R15AI094492-01","title":"Immune Control of Giardia Pathogenesis"},{"funder_name":"National Institutes of Health","grant_id":"2R15AI109591-02A1","title":"The Role of Lectins in Immune Recognition of Giardia."}],"total_grants":6,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"green","license":"Wiley Online Library User Agreement","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7339241","host_type":"repository"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/cpmc.102","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/full-xml/10.1002/cpmc.102","host_type":"publisher"},{"url":"https://currentprotocols.onlinelibrary.wiley.com/doi/am-pdf/10.1002/cpmc.102","host_type":"publisher"},{"url":"https://currentprotocols.onlinelibrary.wiley.com/doi/pdf/10.1002/cpmc.102","host_type":"publisher"},{"url":"https://doi.org/10.1002/cpmc.102","host_type":""},{"url":"https://rss.onlinelibrary.wiley.com/doi/am-pdf/10.1002/cpmc.102","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/32515871","host_type":""},{"url":"https://dx.doi.org/10.1002/cpmc.102","host_type":""}],"fields_of_study":["0301 basic medicine","03 medical and health sciences","0302 clinical medicine","Animals","Cell Culture Techniques","Disease Models, Animal","Giardia lamblia","Giardiasis","Humans","Life Cycle Stages","Mice","Parasitology","Preservation, Biological","Protozoan Proteins","Trophozoites"],"mesh_terms":["Animals","Humans","Mice","Giardia lamblia","Giardiasis","Disease Models, Animal","Protozoan Proteins","Preservation, Biological","Cell Culture Techniques","Parasitology","Life Cycle Stages","Trophozoites"],"keywords":["Cryopreservation","Giardia lamblia","Animal model","Giardiasis","Protozoan Parasite","Murine Infection","Passage And Maintenance","Life Cycle Stages","Preservation, Biological","Cell Culture Techniques","Protozoan Proteins","Disease Models, Animal","Mice","Animals","Humans","Parasitology","Trophozoites"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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