{"doi":"10.1002/cnma.202500301","title":"A Reverse Transcription Polymerase Chain Reaction‐Based Method for Determining Aptamer–Protein Interactions at Nanoparticle Surfaces","abstract":"Nucleic acid–protein interactions influence the activity and stability of DNA‐ and RNA‐functionalized nanomaterials. Herein, a restriction enzyme‐mediated approach coupled with reverse transcription‐polymerase chain reaction (RT‐PCR) for probing aptamer–protein interactions on a nanoparticle (NP) surface as a general method for identifying interactions between proteins and an RNA on a NP is described. This process is enabled by utilizing a restriction enzyme (RE)‐specific sequence at the NP surface that can be masked and subsequently revealed depending on the bound state of a protein at the particle's surface. The method is suitable for investigating a specific protein interaction versus a nonspecific interaction, using an RT‐PCR assay designed to detect exposed nucleic acid ligands at the NP surface. This approach is highly modular and can be applied to a variety of RNA–protein interactions for bioanalytical purposes, including as a selection pressure for in vitro selections and Systematic Evolution of Ligands by EXponential enrichment protocols.","journal":"ChemNanoMat","year":2025,"id":569230,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9523,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":719069,"name":"Shraddha S. Sawant","orcid":"0000-0001-6858-7635","position":1,"is_corresponding":false},{"id":1306417,"name":"Rachelle Canete","orcid":"0009-0007-8653-3164","position":2,"is_corresponding":false},{"id":1469888,"name":"Patrick M. Corrigan","orcid":null,"position":3,"is_corresponding":false},{"id":465789,"name":"Jessica L. Rouge","orcid":"0000-0002-6051-3735","position":4,"is_corresponding":false},{"id":509868,"name":"Saketh Gudipati","orcid":"0000-0002-7886-1260","position":0,"is_corresponding":true}],"reference_count":34,"raw_metadata":null,"created_at":"2026-07-19T02:56:59.652443Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}