{"doi":"10.1002/cne.23355","title":"Tanycyte‐like cells form a blood–cerebrospinal fluid barrier in the circumventricular organs of the mouse brain","abstract":"<jats:title>ABSTRACT</jats:title><jats:p>Tanycytes are highly specialized ependymal cells that form a blood–cerebrospinal fluid (CSF) barrier at the level of the median eminence (ME), a circumventricular organ (CVO) located in the tuberal region of the hypothalamus. This ependymal layer harbors well‐organized tight junctions, a hallmark of central nervous system barriers that is lacking in the fenestrated portal vessels of the ME. The displacement of barrier properties from the vascular to the ventricular side allows the diffusion of blood‐borne molecules into the parenchyma of the ME while tanycyte tight junctions control their diffusion into the CSF, thus maintaining brain homeostasis. In the present work, we combined immunohistochemical and permeability studies to investigate the presence of tanycyte barriers along the ventricular walls of other brain CVOs. Our data indicate that, unlike cuboidal ependymal cells, ependymal cells bordering the CVOs possess long processes that project into the parenchyma of the CVOs to reach the fenestrated capillary network. Remarkably, these tanycyte‐like cells display well‐organized tight junctions around their cell bodies. Consistent with these observations, permeability studies show that this ependymal layer acts as a diffusion barrier. Together, our results suggest that tanycytes are a characteristic feature of all CVOs and yield potential new insights into their involvement in regulating the exchange between the blood, the brain, and the CSF within these “brain windows.” J. Comp. Neurol. 521:3389‐3405, 2013. © 2013 Wiley Periodicals, Inc.</jats:p>","journal":"Journal of Comparative Neurology","year":2013,"id":16106,"datarank":7.636595168419468,"base_score":5.634789603169249,"endowment":5.634789603169249,"self_citation_contribution":0.8452184404753875,"citation_network_contribution":6.791376727944081,"self_endowment_contribution":0.8452184404753875,"citer_contribution":6.791376727944081,"corpus_percentile":null,"corpus_rank":null,"citation_count":279,"citer_count":200,"citers_with_citation_signal":200,"citers_with_endowment":200,"datacite_reuse_total":6,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":119754,"name":"Amandine Mullier","orcid":null,"position":1,"is_corresponding":false},{"id":119755,"name":"Sebastien G. Bouret","orcid":null,"position":2,"is_corresponding":false},{"id":119756,"name":"Vincent Prevot","orcid":null,"position":3,"is_corresponding":false},{"id":120102,"name":"Benedicte Dehouck","orcid":null,"position":4,"is_corresponding":false},{"id":120101,"name":"Fanny Langlet","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":5.634789603169249,"endowment":5.634789603169249,"datacite_reuse_total":6,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23649873","pmcid":"PMC3973970","openalex_id":"https://openalex.org/W1941456052","authors":[],"funders":[{"funder_name":"NIDDK NIH HHS","grant_id":"R01 DK084142","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"DK84142","title":null},{"funder_name":"European Commission","grant_id":"266408","title":"Understanding food-gut-brain mechanisms across the lifespan in the regulation of hunger and satiety for health"},{"funder_name":"National Institutes of Health","grant_id":"5R01DK084142-04","title":"Hormonal and Nutritional Regulation of Hypothalamic Neurogenesis"}],"total_grants":4,"fwci":14.325,"citation_percentile":0.99146641,"influential_citations":18,"citation_trend":[{"year":2013,"count":7},{"year":2014,"count":23},{"year":2015,"count":21},{"year":2016,"count":17},{"year":2017,"count":26},{"year":2018,"count":21},{"year":2019,"count":25},{"year":2020,"count":23},{"year":2021,"count":32},{"year":2022,"count":18},{"year":2023,"count":17},{"year":2024,"count":18},{"year":2025,"count":25},{"year":2026,"count":5}],"oa_status":"green","license":"Wiley Online Library User Agreement","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3973970","host_type":"repository"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/cne.23445","host_type":"GREEN"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3973970","host_type":"repository"},{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcne.23355","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/cne.23355","host_type":"publisher"},{"url":"https://doi.org/10.1002/cne.23355","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/23649873","host_type":"repository"},{"url":"https://inserm.hal.science/inserm-03204458","host_type":"repository"},{"url":"https://doi.org/10.1002/cne.23445","host_type":""},{"url":"https://europepmc.org/articles/pmc3973970?pdf=render","host_type":""},{"url":"https://inserm.hal.science/inserm-03204458v1","host_type":""},{"url":"https://inserm.hal.science/inserm-03204458v1/document","host_type":""},{"url":"https://dx.doi.org/10.1002/cne.23355","host_type":""},{"url":"http://dx.doi.org/10.1002/cne.23445","host_type":""},{"url":"https://doi.org/https://doi.org/10.1002/cne.23355","host_type":""}],"fields_of_study":["Barrier Structure and Function Studies","Neuroscience of respiration and sleep","Neonatal and fetal brain pathology","Biology","Medicine","0301 basic medicine","03 medical and health sciences","0303 health sciences","Animals","Antibodies","Area Postrema","Blood-Brain Barrier","Brain","Cell Membrane Permeability","Cerebral Ventricles","Cerebrospinal Fluid","Ependymoglial Cells","Immunohistochemistry","Male","Median Eminence","Mice","Mice, Inbred C57BL","Permeability","Subcommissural Organ","Subfornical Organ","Tight Junction Proteins"],"mesh_terms":["Animals","Antibodies","Blood-Brain Barrier","Brain","Cell Membrane Permeability","Cerebral Ventricles","Cerebrospinal Fluid","Immunohistochemistry","Male","Median Eminence","Mice, Inbred C57BL","Permeability","Subcommissural Organ","Subfornical Organ","Area Postrema","Mice","Tight Junction Proteins","Ependymoglial Cells"],"keywords":["Circumventricular organs","Ependymal Cell","Subcommissural organ","Tight junction","Subfornical organ","Blood–brain barrier","Cerebrospinal fluid","Median eminence","Pia mater","Parenchyma","Area postrema","Biology","Hypothalamus","Anatomy","Neuroscience","Pathology","Central nervous system","Cell biology","Medicine","Endocrinology","Tight Junction Protein","Ependymocytes","Organum Vasculosum Laminae Terminalis","Male","Cell Membrane Permeability","Ependymoglial Cells","530","Antibodies","Permeability","Cerebral Ventricles","Mice","616","Animals","[SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]","Tight Junction Proteins","Brain","tight junction protein.","Immunohistochemistry","Mice, Inbred C57BL","Blood-Brain Barrier","[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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