{"doi":"10.1002/ceat.200500144","title":"Protein Engineering Strategies for Selective Protein Purification","abstract":"<jats:title>Abstract</jats:title><jats:p>When producing and purifying recombinant proteins it is of importance to minimize the number of unit operations during the purification procedure. This is accomplished by increasing the selectivity in each step. Due to the high selectivity of affinity chromatography it has a widespread use in protein purification. However, most target proteins lack a suitable affinity ligand usable for capture on a solid matrix. A way to circumvent this obstacle is to genetically fuse the gene encoding the target protein with a gene encoding a purification tag. When the chimeric protein is expressed, the tag allows for specific capture of the fusion protein. In industrial‐scale production, extension of the target protein often is unwanted since it might interfere with the function of the target protein. Hence, a purification scheme developed for the native protein is desired. In this review, different fusion strategies used for protein purification are discussed. Also, the development of ligands for selective affinity purification of native target proteins is surveyed.</jats:p>","journal":"Chemical Engineering &amp; Technology","year":2005,"id":15029,"datarank":2.434839773745849,"base_score":3.9512437185814275,"endowment":3.9512437185814275,"self_citation_contribution":0.5926865577872142,"citation_network_contribution":1.8421532159586347,"self_endowment_contribution":0.5926865577872142,"citer_contribution":1.8421532159586347,"corpus_percentile":null,"corpus_rank":null,"citation_count":51,"citer_count":38,"citers_with_citation_signal":35,"citers_with_endowment":35,"datacite_reuse_total":4,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":116198,"name":"T. Gräslund","orcid":null,"position":1,"is_corresponding":false},{"id":116199,"name":"S. Hober","orcid":null,"position":2,"is_corresponding":false},{"id":116197,"name":"M. Hedhammar","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.9512437185814275,"endowment":3.9512437185814275,"datacite_reuse_total":4,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21071399","pmcid":null,"openalex_id":"https://openalex.org/W2130171154","authors":[],"funders":[],"total_grants":0,"fwci":2.6,"citation_percentile":0.89158496,"influential_citations":1,"citation_trend":[{"year":2012,"count":7},{"year":2013,"count":2},{"year":2014,"count":6},{"year":2016,"count":6},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2020,"count":1},{"year":2022,"count":1},{"year":2023,"count":3},{"year":2024,"count":2},{"year":2025,"count":1}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fceat.200500144","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/ceat.200500144","host_type":"publisher"},{"url":"https://doi.org/10.1002/ceat.200500144","host_type":"journal"}],"fields_of_study":["Monoclonal and Polyclonal Antibodies Research","Protein purification and stability","Viral Infectious Diseases and Gene Expression in Insects","Chemistry","Engineering"],"mesh_terms":[],"keywords":["Tandem affinity purification","FLAG-tag","Target protein","Protein purification","Affinity chromatography","Fusion protein","Myc-tag","Protein engineering","Protein tag","Chemistry","Ligand (biochemistry)","Recombinant DNA","Biochemistry","Chromatography","Gene"],"sdg_mappings":[],"linked_datasets":[{"doi":"10.6084/m9.figshare.24457711.v1","title":"Additional file 1 of A novel protein purification scheme based on salt inducible self-assembling peptides","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.24457711","title":"Additional file 1 of A novel protein purification scheme based on salt inducible self-assembling peptides","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.24458002.v1","title":"Additional file 2 of A novel protein purification scheme based on salt inducible self-assembling peptides","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.24458002","title":"Additional file 2 of A novel protein purification scheme based on salt inducible self-assembling peptides","publisher":"figshare","resource_type":"JournalArticle"}],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-01T16:04:52.551700Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}