{"doi":"10.1002/cbic.202000268","title":"Efficient Chemoenzymatic Synthesis of N‐Glycans with a β1,4‐Galactosylated Bisecting GlcNAc Motif","abstract":"In human serum immunoglobulin G (IgG), a rare modification of biantennary complex N-glycans lead to a β1,4-galactosylated bisecting GlcNAc branch. We found that the bisecting GlcNAc on a biantennary core-fucosylated N-glycan was enzymatically galactosylated under stringent reaction conditions. Further optimizations led to an efficient enzymatic approach to this particular modification for biantennary substrates. Notably, tri- and tetra-antennary complex N-glycans were not converted by bovine galactosyltransferase. An N-glycan with a galactosylated bisecting GlcNAc was linked to a lanthanide binding tag. The pseudo-contact shifts (PCS) obtained from the corresponding Dy-complex were used to calculate the conformational preferences of the rare N-glycan. Besides two extended conformations only a single folded conformation was found.","journal":"ChemBioChem","year":2020,"id":78756,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":17,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9464,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":410584,"name":"Dimitri Ott","orcid":null,"position":1,"is_corresponding":false},{"id":409506,"name":"Theodoros Karagiannis","orcid":"0009-0008-6029-3753","position":2,"is_corresponding":false},{"id":410585,"name":"Markus Weishaupt","orcid":null,"position":3,"is_corresponding":false},{"id":410586,"name":"Mathäus Niemietz","orcid":null,"position":4,"is_corresponding":false},{"id":410587,"name":"Steffen Eller","orcid":null,"position":5,"is_corresponding":false},{"id":249284,"name":"Marie T. Lott","orcid":"0000-0003-2035-479X","position":6,"is_corresponding":false},{"id":409507,"name":"Mónica Martínez‐Orts","orcid":"0000-0002-9098-9101","position":7,"is_corresponding":false},{"id":409508,"name":"Ángeles Canales","orcid":"0000-0003-0542-3080","position":8,"is_corresponding":false},{"id":410588,"name":"Nahid Razi","orcid":null,"position":9,"is_corresponding":false},{"id":230746,"name":"James C. Paulson","orcid":"0000-0003-4589-5322","position":10,"is_corresponding":false},{"id":409509,"name":"Carlo Unverzagt","orcid":"0000-0001-6492-1747","position":11,"is_corresponding":false},{"id":410583,"name":"Michael Weiß","orcid":null,"position":0,"is_corresponding":true}],"reference_count":27,"raw_metadata":null,"created_at":"2026-07-18T21:50:09.793321Z","pmid":"32597008","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}