{"doi":"10.1002/cbic.201700107","title":"Aromatic Polyketide GTRI‐02 is a Previously Unidentified Product of the <i>act</i> Gene Cluster in <i>Streptomyces coelicolor</i> A3(2)","abstract":"<jats:title>Abstract</jats:title><jats:p>The biosynthesis of aromatic polyketides derived from type II polyketide synthases (PKSs) is complex, and it is not uncommon that highly similar gene clusters give rise to diverse structural architectures. The <jats:italic>act</jats:italic> biosynthetic gene cluster (BGC) of the model actinomycete <jats:italic>Streptomyces coelicolor</jats:italic> A3(2) is an archetypal type II PKS. Here we show that the <jats:italic>act</jats:italic> BGC also specifies the aromatic polyketide GTRI‐02 (<jats:bold>1</jats:bold>) and propose a mechanism for the biogenesis of its 3,4‐dihydronaphthalen‐1(2<jats:italic>H</jats:italic>)‐one backbone. Polyketide <jats:bold>1</jats:bold> was also produced by <jats:italic>Streptomyces</jats:italic> sp. MBT76 after activation of the <jats:italic>act</jats:italic>‐like <jats:italic>qin</jats:italic> gene cluster by overexpression of the pathway‐specific activator. Mining of this strain also identified dehydroxy‐GTRI‐02 (<jats:bold>2</jats:bold>), which most likely originated from dehydration of <jats:bold>1</jats:bold> during the isolation process. This work shows that even extensively studied model gene clusters such as <jats:italic>act</jats:italic> of <jats:italic>S. coelicolor</jats:italic> can still produce new chemistry, offering new perspectives for drug discovery.</jats:p>","journal":"ChemBioChem","year":2017,"id":682563,"datarank":0.49983067652628066,"base_score":3.332204510175204,"endowment":3.332204510175204,"self_citation_contribution":0.49983067652628066,"citation_network_contribution":0.0,"self_endowment_contribution":0.49983067652628066,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":27,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1598317,"name":"Koji Ichinose","orcid":null,"position":1,"is_corresponding":false},{"id":1783164,"name":"Young Hae Choi","orcid":null,"position":2,"is_corresponding":false},{"id":274283,"name":"Gilles P. van Wezel","orcid":"0000-0003-0341-1561","position":3,"is_corresponding":false},{"id":282651,"name":"Changsheng Wu","orcid":"0000-0002-0921-6356","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Aromatic Polyketide GTRI‐02 is a Previously Unidentified Product of the <i>act</i> Gene Cluster in <i>Streptomyces coelicolor</i> A3(2)","abstract":"<jats:title>Abstract</jats:title><jats:p>The biosynthesis of aromatic polyketides derived from type II polyketide synthases (PKSs) is complex, and it is not uncommon that highly similar gene clusters give rise to diverse structural architectures. The <jats:italic>act</jats:italic> biosynthetic gene cluster (BGC) of the model actinomycete <jats:italic>Streptomyces coelicolor</jats:italic> A3(2) is an archetypal type II PKS. Here we show that the <jats:italic>act</jats:italic> BGC also specifies the aromatic polyketide GTRI‐02 (<jats:bold>1</jats:bold>) and propose a mechanism for the biogenesis of its 3,4‐dihydronaphthalen‐1(2<jats:italic>H</jats:italic>)‐one backbone. Polyketide <jats:bold>1</jats:bold> was also produced by <jats:italic>Streptomyces</jats:italic> sp. MBT76 after activation of the <jats:italic>act</jats:italic>‐like <jats:italic>qin</jats:italic> gene cluster by overexpression of the pathway‐specific activator. Mining of this strain also identified dehydroxy‐GTRI‐02 (<jats:bold>2</jats:bold>), which most likely originated from dehydration of <jats:bold>1</jats:bold> during the isolation process. This work shows that even extensively studied model gene clusters such as <jats:italic>act</jats:italic> of <jats:italic>S. coelicolor</jats:italic> can still produce new chemistry, offering new perspectives for drug discovery.</jats:p>","is_dataset_classified":null,"base_score":3.332204510175204,"endowment":3.332204510175204,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"28463421","pmcid":null,"openalex_id":"https://openalex.org/W2611628383","authors":[],"funders":[{"funder_name":"China Scholarship Council","grant_id":"","title":null}],"total_grants":1,"fwci":1.7091,"citation_percentile":0.8242627,"influential_citations":0,"citation_trend":[{"year":2018,"count":3},{"year":2019,"count":4},{"year":2020,"count":2},{"year":2021,"count":5},{"year":2022,"count":3},{"year":2023,"count":4},{"year":2024,"count":1},{"year":2025,"count":5}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcbic.201700107","host_type":"publisher"},{"url":"https://chemistry-europe.onlinelibrary.wiley.com/doi/pdf/10.1002/cbic.201700107","host_type":"publisher"},{"url":"https://doi.org/10.1002/cbic.201700107","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/28463421","host_type":"repository"},{"url":"https://hdl.handle.net/1887/3192000","host_type":"repository"},{"url":"http://hdl.handle.net/1887/3192000","host_type":"repository"}],"fields_of_study":["Microbial Natural Products and Biosynthesis","Fungal Biology and Applications","Plant-Microbe Interactions and Immunity"],"mesh_terms":["Multigene Family","Naphthols","Tetrahydronaphthalenes","Streptomyces coelicolor"],"keywords":["Streptomyces coelicolor","Polyketide","Gene cluster","Streptomyces","Gene","Biology","Streptomycetaceae","Biogenesis","Polyketide synthase","Genetics","Computational biology","Biosynthesis","Actinomycetales","Mutant","Antibiotics","Polyketides","Nmr Profiling","Type ii Pkss"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T20:45:20.883546Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}