{"doi":"10.1002/cbic.201300033","title":"A Highly Efficient Molecular Cloning Platform that Utilises a Small Bacterial Toxin Gene","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>\n                    Molecular cloning technologies that have emerged in recent years are more efficient and simpler to use than traditional strategies, but many have the disadvantages of requiring multiple steps and expensive proprietary enzymes. We have engineered cloning vectors containing variants of IbsC, a 19‐residue toxin from\n                    <jats:italic>Escherichia coli</jats:italic>\n                    K‐12. These toxic peptides offer selectivity to minimise the background, labour, and cost associated with conventional molecular cloning. As demonstrated with the cloning of reporter genes, this “detox cloning” system consistently produced over 95 % positive clones. Purification steps between digestion and ligation are not necessary, and the total time between digestion and plating of transformants can be as little as three hours. Thus, these IbsC‐based cloning vectors are as reliable and amenable to high‐throughput cloning as commercially available systems, and have the advantage of being more time‐efficient and cost‐effective.\n                  </jats:p>","journal":"ChemBioChem","year":2013,"id":19510,"datarank":0.9183586058858284,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.5456226084176283,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.5456226084176283,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":11,"citers_with_citation_signal":10,"citers_with_endowment":10,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":131968,"name":"Yingfu Li","orcid":null,"position":1,"is_corresponding":false},{"id":131967,"name":"Wendy W. K. Mok","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23512843","pmcid":null,"openalex_id":"https://openalex.org/W2103537794","authors":[],"funders":[],"total_grants":0,"fwci":1.1459,"citation_percentile":0.80974407,"influential_citations":0,"citation_trend":[{"year":2013,"count":1},{"year":2014,"count":2},{"year":2015,"count":1},{"year":2016,"count":1},{"year":2019,"count":1},{"year":2020,"count":2},{"year":2022,"count":1},{"year":2023,"count":1}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcbic.201300033","host_type":"publisher"},{"url":"https://chemistry-europe.onlinelibrary.wiley.com/doi/pdf/10.1002/cbic.201300033","host_type":"publisher"},{"url":"https://doi.org/10.1002/cbic.201300033","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/23512843","host_type":"repository"}],"fields_of_study":["Bacterial Genetics and Biotechnology","Bacteriophages and microbial interactions","RNA and protein synthesis mechanisms","Biology","Medicine","Chemistry","Amino Acid Sequence","Bacterial Toxins","Base Sequence","Cloning, Molecular","Escherichia coli K12","Escherichia coli Proteins","Genes, Bacterial","Genes, Reporter","Genetic Vectors","High-Throughput Screening Assays","Molecular Sequence Data"],"mesh_terms":["Amino Acid Sequence","Bacterial Toxins","Base Sequence","Cloning, Molecular","Genes, Bacterial","Genetic Vectors","Molecular Sequence Data","Genes, Reporter","Escherichia coli Proteins","Escherichia coli K12","High-Throughput Screening Assays"],"keywords":["Cloning (programming)","Molecular cloning","Computational biology","Escherichia coli","Biology","Gene","Molecular biology","Chemistry","Genetics","Computer science","Gene expression"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Decent work and economic growth"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-04T04:30:34.505999Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}