{"doi":"10.1002/btm2.10689","title":"Single immunization with an influenza <scp>hemagglutinin</scp> nanoparticle‐based vaccine elicits durable protective immunity","abstract":"Vaccination is the most effective strategy to combat influenza. Ideally, potent and persistent vaccine effects would be induced with a single vaccine dose. Here, we designed a virus-like particle (VLP)-based vaccine presenting multiple copies of the influenza hemagglutinin (HA) from A/Puerto Rico/8/1934 (PR8HA-VLP) and examined its immunogenicity and protective efficacy in ferrets. Serum-neutralizing antibodies were effectively induced against the homologous virus at 3-week post-vaccination with a single dose of PR8HA-VLP with or without adjuvants. When the single-immunized ferrets were challenged with the homologous virus, virus replication in the nasal mucosa was significantly reduced. Long-term monitoring of serum titers revealed that after adjuvanted vaccination with PR8HA-VLP, neutralizing antibodies were retained at similar levels 20- to 183-week post-vaccination, although a 4- to 8-fold titer decline was observed from 3- to 20-week post-vaccination. Boost immunization at 183 weeks after the first immunization elicited higher neutralizing antibody titers than those at 3 weeks after the initial immunization in most of the animals. These results confirm that nanoparticle-based vaccines are a promising approach to effectively elicit durable multiyear neutralizing antibody responses against influenza viruses.","journal":"Bioengineering & Translational Medicine","year":2024,"id":457741,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.951,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":616891,"name":"Tadashi Maemura","orcid":null,"position":1,"is_corresponding":false},{"id":858617,"name":"Kathryn Loeffler","orcid":"0009-0005-4935-5819","position":2,"is_corresponding":false},{"id":858616,"name":"Steven J. Frey","orcid":"0000-0003-1863-694X","position":3,"is_corresponding":false},{"id":1133284,"name":"Chunyang Gu","orcid":"0000-0003-1283-1701","position":4,"is_corresponding":false},{"id":1283274,"name":"Asim Biswas","orcid":"0000-0002-4999-4697","position":5,"is_corresponding":false},{"id":125952,"name":"Masato Hatta","orcid":null,"position":6,"is_corresponding":false},{"id":107110,"name":"Yoshihiro Kawaoka","orcid":"0000-0001-5061-8296","position":7,"is_corresponding":false},{"id":305337,"name":"Ravi S. Kane","orcid":"0000-0003-3084-4098","position":8,"is_corresponding":false},{"id":456507,"name":"S. Chiba","orcid":"0000-0003-0415-6013","position":0,"is_corresponding":true}],"reference_count":28,"raw_metadata":null,"created_at":"2026-07-19T02:03:41.744499Z","pmid":"39553436","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}