{"doi":"10.1002/btm2.10285","title":"Monocytes engineered with <scp>iSNAP</scp> inhibit human <scp>B‐lymphoma</scp> progression","abstract":"Monocytes are important regulators for the maintenance of homeostasis in innate and adaptive immune system and have been reported to play important role in cancer progression. CD47-SIRPα recognition is a coinhibitory immune signal to inhibit phagocytosis in monocytes and macrophages and has been well-known as the \"Don't eat me\" signal. By using an approach of integrated sensing and activating proteins (iSNAPs), we have rewired the CD47-SIRPα axis to create iSNAP-M which activates pathways in engineered human monocytes (iSNAP-MC). The mRNA expression levels of the monocyte/macrophage markers CD11b, CD14, and CD31 are upregulated in iSNAP-monocytes (iSNAP-MC). With PMA induction, the iSNAP-MC-derived macrophages (iSNAP-MΦ) showed upregelation in CD86 and CD80, but not CD206. TNFα expression and secretion were also increased in iSNAP-MΦ. Furthermore, the injection of iSNAP-MC into mice bearing human B-lymphoma tumors led to the suppression of tumor progression. Therefore, the engineered monocytes, via blockage of coinhibitory immune signals by rewiring CD47-SIRPα axis, can be applied to suppress target tumors for cancer immunotherapy.","journal":"Bioengineering & Translational Medicine","year":2021,"id":208023,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9541,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":432271,"name":"Siamak Amirfakhri","orcid":"0000-0002-5534-1043","position":1,"is_corresponding":false},{"id":794078,"name":"Hsin‐Hung Lin","orcid":"0000-0001-8327-6835","position":2,"is_corresponding":false},{"id":227846,"name":"Hannah M. Hollandsworth","orcid":"0000-0002-8867-6835","position":3,"is_corresponding":false},{"id":429219,"name":"Filemoni Filemoni","orcid":null,"position":4,"is_corresponding":false},{"id":621876,"name":"Yahan Liu","orcid":"0000-0002-5948-4615","position":5,"is_corresponding":false},{"id":254297,"name":"Yiqian Wu","orcid":"0000-0002-6313-6015","position":6,"is_corresponding":false},{"id":794564,"name":"Julie Y.-S. Li","orcid":null,"position":7,"is_corresponding":false},{"id":794079,"name":"Hongquan Xu","orcid":"0000-0002-3502-0664","position":8,"is_corresponding":false},{"id":254302,"name":"Shu Chien","orcid":"0000-0003-0332-285X","position":9,"is_corresponding":false},{"id":399592,"name":"Michael Bouvet","orcid":"0000-0002-0086-2159","position":10,"is_corresponding":false},{"id":254303,"name":"Yingxiao Wang","orcid":"0000-0003-0265-326X","position":11,"is_corresponding":false},{"id":525601,"name":"Hao-Hsiang Wu","orcid":"0000-0001-9665-8672","position":0,"is_corresponding":true}],"reference_count":44,"raw_metadata":null,"created_at":"2026-07-18T23:51:53.662668Z","pmid":"35600645","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}