{"doi":"10.1002/bmc.5169","title":"Pharmacokinetic study of an anti‐trypanosome agent with different formulations and administration routes in mice by HPLC–MS/MS","abstract":"Abstract Previously compound 12 showed great anti‐trypanosome activity without toxicity in an in vivo study. In the current study, a sensitive and rapid high‐performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) method was developed and validated to investigate its pharmacokinetics in mouse plasma. A protein precipitation method was applied to extract the compound, and it was then separated using a Kinetex C 18 column with mobile phase consisting of acetonitrile–0.1% formic acid water (50:50, v/v) at a flow rate of 300 μl/min. The analytes were detected with the multiple reaction monitoring in negative electrospray ionization source for quantitative response of the compounds. Compound 12 was detected at m/z 477.0 → 367.2, while the internal standard compound 14 was detected at m/z 499.2 → 268.2. Inter‐ and intra‐day precision was <5.22 and 2.79% respectively, while the accuracy range was within ±9.65%. The method was successfully applied to evaluate the pharmacokinetics of compound 12 in mouse plasma with two formulations (20% Cremophor EL or sesame oil) and drug administration routes (oral and intraperitoneal injection). We observed a better drug serum concentration with the Cremophor formulation, and the two different drug administration routes did not show significant differences from the drug distribution.","journal":"Biomedical Chromatography","year":2021,"id":210234,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.945,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":513650,"name":"Cody M. Orahoske","orcid":"0000-0001-8427-8891","position":1,"is_corresponding":false},{"id":798774,"name":"Raina Dano","orcid":null,"position":2,"is_corresponding":false},{"id":798367,"name":"Wenjing Zhang","orcid":"0000-0003-2615-8976","position":3,"is_corresponding":false},{"id":410131,"name":"Bibo Li","orcid":"0000-0001-5804-9876","position":4,"is_corresponding":false},{"id":513656,"name":"Bin Su","orcid":"0000-0002-0711-441X","position":5,"is_corresponding":false},{"id":798366,"name":"Yaxin Li","orcid":"0000-0001-7550-0710","position":0,"is_corresponding":true}],"reference_count":18,"raw_metadata":null,"created_at":"2026-07-18T23:52:08.869117Z","pmid":"33978959","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}