{"doi":"10.1002/bmc.1164","title":"A sensitive and specific liquid chromatography/tandem mass spectrometry method for determination of echinacoside and its pharmacokinetic application in rats","abstract":"<jats:title>Abstract</jats:title><jats:p>A rapid and sensitive method based on liquid chromatography/tandem mass spectrometry (LC/MS/MS) for the determination of echinacoside in rat plasma was established and fully validated. A single step of liquid–liquid extraction with <jats:italic>n</jats:italic>‐butanol was utilized. Chromatographic separation of the analyte and the internal standard (IS), chlorogenic acid, from the sample matrix was performed using a Capcell‐MG C<jats:sub>18</jats:sub> analytical column (100 2.0 mm × 5 µm), with a gradient of acetonitrile and water containing 0.1% acetic acid as the mobile phase. Detection was performed on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization source operated in negative ion selected reaction monitoring mode. The method was linear in the concentration range 10–2500 ng/mL. The deviations of both intra‐ and inter‐day precisions (RSD) were 7.1% and the assay accuracies were within 99.2–106.5%. Echinacoside proved to be stable during sample storage, preparation and analysis when an antioxidant solution was used. The method was successfully applied to a pharmacokinetic study in rats after an intragastric administration of echinacoside (100 mg/kg). With the lower limit of quantification at 10 ng/mL, this method proved to have sufficient selectivity, sensitivity and reproducibility for the pharmacokinetic study of echinacoside. Copyright © 2009 John Wiley &amp; Sons, Ltd.</jats:p>","journal":"Biomedical Chromatography","year":2009,"id":631812,"datarank":1.1276054333271417,"base_score":2.772588722239781,"endowment":2.772588722239781,"self_citation_contribution":0.41588830833596724,"citation_network_contribution":0.7117171249911745,"self_endowment_contribution":0.41588830833596724,"citer_contribution":0.7117171249911745,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":14,"citers_with_citation_signal":11,"citers_with_endowment":11,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":678646,"name":"Guangji Wang","orcid":"0000-0002-0777-3896","position":1,"is_corresponding":false},{"id":678645,"name":"Haiping Hao","orcid":"0000-0003-2522-7546","position":2,"is_corresponding":false},{"id":1637475,"name":"Pengfei Tu","orcid":null,"position":3,"is_corresponding":false},{"id":737378,"name":"Yong Jiang","orcid":"0000-0002-1198-1807","position":4,"is_corresponding":false},{"id":180341,"name":"Qiong Wang","orcid":null,"position":5,"is_corresponding":false},{"id":331988,"name":"Yan Zhang","orcid":"0000-0001-8934-7016","position":6,"is_corresponding":false},{"id":478625,"name":"Chaonan Zheng","orcid":"0009-0004-4092-861X","position":7,"is_corresponding":false},{"id":202136,"name":"Yuxin Wang","orcid":null,"position":8,"is_corresponding":false},{"id":1142191,"name":"Liang Dai","orcid":"0000-0003-1053-7189","position":9,"is_corresponding":false},{"id":1246361,"name":"Hao Yang","orcid":"0000-0003-2214-9474","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A sensitive and specific liquid chromatography/tandem mass spectrometry method for determination of echinacoside and its pharmacokinetic application in rats","abstract":"<jats:title>Abstract</jats:title><jats:p>A rapid and sensitive method based on liquid chromatography/tandem mass spectrometry (LC/MS/MS) for the determination of echinacoside in rat plasma was established and fully validated. A single step of liquid–liquid extraction with <jats:italic>n</jats:italic>‐butanol was utilized. Chromatographic separation of the analyte and the internal standard (IS), chlorogenic acid, from the sample matrix was performed using a Capcell‐MG C<jats:sub>18</jats:sub> analytical column (100 2.0 mm × 5 µm), with a gradient of acetonitrile and water containing 0.1% acetic acid as the mobile phase. Detection was performed on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization source operated in negative ion selected reaction monitoring mode. The method was linear in the concentration range 10–2500 ng/mL. The deviations of both intra‐ and inter‐day precisions (RSD) were 7.1% and the assay accuracies were within 99.2–106.5%. Echinacoside proved to be stable during sample storage, preparation and analysis when an antioxidant solution was used. The method was successfully applied to a pharmacokinetic study in rats after an intragastric administration of echinacoside (100 mg/kg). With the lower limit of quantification at 10 ng/mL, this method proved to have sufficient selectivity, sensitivity and reproducibility for the pharmacokinetic study of echinacoside. Copyright © 2009 John Wiley &amp; Sons, Ltd.</jats:p>","is_dataset_classified":null,"base_score":2.772588722239781,"endowment":2.772588722239781,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19277965","pmcid":null,"openalex_id":"https://openalex.org/W2062375825","authors":[],"funders":[],"total_grants":0,"fwci":1.8431,"citation_percentile":0.89967785,"influential_citations":0,"citation_trend":[{"year":2013,"count":2},{"year":2014,"count":1},{"year":2016,"count":1},{"year":2018,"count":1},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2021,"count":3},{"year":2023,"count":1},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbmc.1164","host_type":"publisher"},{"url":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/pdf/10.1002/bmc.1164","host_type":"publisher"},{"url":"https://doi.org/10.1002/bmc.1164","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/19277965","host_type":"repository"}],"fields_of_study":["Herbal Medicine Research Studies","Phytochemistry and Biological Activities","Toxin Mechanisms and Immunotoxins"],"mesh_terms":["Animals","Chlorogenic Acid","Chromatography, Liquid","Glycosides","Male","Sensitivity and Specificity","Time Factors","Rats, Sprague-Dawley","1-Butanol","Spectrometry, Mass, Electrospray Ionization","Rats","Tandem Mass Spectrometry"],"keywords":["Chemistry","Chromatography","Selected reaction monitoring","Analyte","Triple quadrupole mass spectrometer","Electrospray ionization","Tandem mass spectrometry","Mass spectrometry","Liquid chromatography–mass spectrometry","Detection limit","Extraction (chemistry)","Sample preparation","Electrospray","Analytical Chemistry (journal)"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T00:46:06.115890Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}