{"doi":"10.1002/bmb.21514","title":"A guided‐inquiry investigation of genetic variants using Oxford nanopore sequencing for an undergraduate molecular biology laboratory course","abstract":"Next Generation Sequencing (NGS) has become an important tool in the biological sciences and has a growing number of applications across medical fields. Currently, few undergraduate programs provide training in the design and implementation of NGS applications. Here, we describe an inquiry-based laboratory exercise for a college-level molecular biology laboratory course that uses real-time MinION deep sequencing and bioinformatics to investigate characteristic genetic variants found in cancer cell-lines. The overall goal for students was to identify non-small cell lung cancer (NSCLC) cell-lines based on their unique genomic profiles. The units described in this laboratory highlight core principles in multiplex PCR primer design, real-time deep sequencing, and bioinformatics analysis for genetic variants. We found that the MinION device is an appropriate, feasible tool that provides a comprehensive, hands-on NGS experience for undergraduates. Student evaluations demonstrated increased confidence in using molecular techniques and enhanced understanding of NGS concepts. Overall, this exercise provides a pedagogical tool for incorporating NGS approaches in the teaching laboratory as way of enhancing students' comprehension of genomic sequence analysis. Further, this NGS lab module can easily be added to a variety of lab-based courses to help undergraduate students learn current DNA sequencing methods with limited effort and cost.","journal":"Biochemistry and Molecular Biology Education","year":2021,"id":208249,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9507,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":766630,"name":"Michael Mariani","orcid":"0000-0001-5852-0517","position":1,"is_corresponding":false},{"id":725205,"name":"Jamie C. Gay","orcid":null,"position":2,"is_corresponding":false},{"id":395948,"name":"Tyler Hogan","orcid":"0000-0003-4718-0726","position":3,"is_corresponding":false},{"id":395949,"name":"Eyal Amiel","orcid":"0000-0002-1578-8705","position":4,"is_corresponding":false},{"id":666494,"name":"Paula B. Deming","orcid":"0000-0003-3278-1705","position":5,"is_corresponding":false},{"id":75998,"name":"Seth Frietze","orcid":"0000-0003-4058-3661","position":6,"is_corresponding":false},{"id":395404,"name":"Princess D. Rodriguez","orcid":"0009-0003-0790-9890","position":0,"is_corresponding":true}],"reference_count":22,"raw_metadata":null,"created_at":"2026-07-18T23:51:53.662668Z","pmid":"33939256","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}