{"doi":"10.1002/bit.22831","title":"Farnesol production from <i>Escherichia coli</i> by harnessing the exogenous mevalonate pathway","abstract":"<jats:title>Abstract</jats:title><jats:p>Farnesol (FOH) production has been carried out in metabolically engineered <jats:italic>Escherichia coli</jats:italic>. FOH is formed through the depyrophosphorylation of farnesyl pyrophosphate (FPP), which is synthesized from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) by FPP synthase. In order to increase FPP synthesis, <jats:italic>E. coli</jats:italic> was metabolically engineered to overexpress <jats:italic>ispA</jats:italic> and to utilize the foreign mevalonate (MVA) pathway for the efficient synthesis of IPP and DMAPP. Two‐phase culture using a decane overlay of the culture broth was applied to reduce volatile loss of FOH produced during culture and to extract FOH from the culture broth. A FOH production of 135.5 mg/L was obtained from the recombinant <jats:italic>E. coli</jats:italic> harboring the pTispA and pSNA plasmids for <jats:italic>ispA</jats:italic> overexpression and MVA pathway utilization, respectively. It is interesting to observe that a large amount of FOH could be produced from <jats:italic>E. coli</jats:italic> without FOH synthase by the augmentation of FPP synthesis. Introduction of the exogenous MVA pathway enabled the dramatic production of FOH by <jats:italic>E. coli</jats:italic> while no detectable FOH production was observed in the endogenous MEP pathway‐only control. Biotechnol. Bioeng. 2010;107: 421–429. © 2010 Wiley Periodicals, Inc.</jats:p>","journal":"Biotechnology and Bioengineering","year":2010,"id":607076,"datarank":0.7143260902196635,"base_score":4.762173934797756,"endowment":4.762173934797756,"self_citation_contribution":0.7143260902196635,"citation_network_contribution":0.0,"self_endowment_contribution":0.7143260902196635,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":116,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1558690,"name":"Sang‐Hwal Yoon","orcid":null,"position":1,"is_corresponding":false},{"id":1558693,"name":"Asad Ali Shah","orcid":null,"position":2,"is_corresponding":false},{"id":1558694,"name":"Young‐Ryun Chung","orcid":null,"position":3,"is_corresponding":false},{"id":1558695,"name":"Jae‐Yean Kim","orcid":null,"position":4,"is_corresponding":false},{"id":1558696,"name":"Eui‐Sung Choi","orcid":null,"position":5,"is_corresponding":false},{"id":208770,"name":"Jay D. 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In order to increase FPP synthesis, <jats:italic>E. coli</jats:italic> was metabolically engineered to overexpress <jats:italic>ispA</jats:italic> and to utilize the foreign mevalonate (MVA) pathway for the efficient synthesis of IPP and DMAPP. Two‐phase culture using a decane overlay of the culture broth was applied to reduce volatile loss of FOH produced during culture and to extract FOH from the culture broth. A FOH production of 135.5 mg/L was obtained from the recombinant <jats:italic>E. coli</jats:italic> harboring the pTispA and pSNA plasmids for <jats:italic>ispA</jats:italic> overexpression and MVA pathway utilization, respectively. It is interesting to observe that a large amount of FOH could be produced from <jats:italic>E. coli</jats:italic> without FOH synthase by the augmentation of FPP synthesis. Introduction of the exogenous MVA pathway enabled the dramatic production of FOH by <jats:italic>E. coli</jats:italic> while no detectable FOH production was observed in the endogenous MEP pathway‐only control. Biotechnol. 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