{"doi":"10.1002/biot.202400070","title":"Microfabricated dynamic brain organoid cocultures to assess the effects of surface geometry on assembloid formation","abstract":"<jats:title>Abstract</jats:title><jats:p>Organoids have emerged as valuable tools for the study of development and disease. Assembloids are formed by integrating multiple organoid types to create more complex models. However, the process by which organoids integrate to form assembloids remains unclear and may play an important role in the resulting organoid structure. Here, a microfluidic platform is developed that allows separate culture of distinct organoid types and provides the capacity to partially control the geometry of the resulting organoid surfaces. Removal of a microfabricated barrier then allows the shaped and positioned organoids to interact and form an assembloid. When midbrain and unguided brain organoids were allowed to assemble with a defined spacing between them, axonal projections from midbrain organoids and cell migration out of unguided organoids were observed and quantitatively measured as the two types of organoids fused together. Axonal projection directions were statistically biased toward other midbrain organoids, and unguided organoid surface geometry was found to affect cell invasion. This platform provides a tool to observe cellular interactions between organoid surfaces that are spaced apart in a controlled manner, and may ultimately have value in exploring neuronal migration, axon targeting, and assembloid formation mechanisms.</jats:p>","journal":"Biotechnology Journal","year":2024,"id":17086,"datarank":0.3775778448358924,"base_score":2.302585092994046,"endowment":2.302585092994046,"self_citation_contribution":0.3453877639491069,"citation_network_contribution":0.03219008088678549,"self_endowment_contribution":0.3453877639491069,"citer_contribution":0.03219008088678549,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":7,"citers_with_citation_signal":4,"citers_with_endowment":4,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":123489,"name":"Sabra Rostami","orcid":null,"position":1,"is_corresponding":false},{"id":123490,"name":"Vanessa Xu","orcid":null,"position":2,"is_corresponding":false},{"id":60427,"name":"Chen Li","orcid":"0000-0002-1847-754X","position":3,"is_corresponding":false},{"id":123491,"name":"Paula Lépine","orcid":null,"position":4,"is_corresponding":false},{"id":123492,"name":"Thomas M. Durcan","orcid":null,"position":5,"is_corresponding":false},{"id":123493,"name":"Christopher Moraes","orcid":"0000-0002-8950-2212","position":6,"is_corresponding":false},{"id":123488,"name":"Camille Cassel de Camps","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"39167558","pmcid":null,"openalex_id":"https://openalex.org/W4401794395","authors":[],"funders":[{"funder_name":"Natural Sciences and Engineering Research Council of Canada","grant_id":"RGPIN‐2022‐05165","title":null},{"funder_name":"Natural Sciences and Engineering Research Council of Canada","grant_id":"RGPIN-2022-05165","title":null},{"funder_name":"Natural Sciences and Engineering Research Council of Canada","grant_id":"unidentified","title":"unidentified"},{"funder_name":"Alain and Sandra Bouchard Foundation","grant_id":"","title":null},{"funder_name":"Chamandy Foundation","grant_id":"","title":null},{"funder_name":"Québec Consortium for Drug Discovery","grant_id":"","title":null},{"funder_name":"Canada First Research Excellence Fund","grant_id":"","title":null},{"funder_name":"Canada Research Chairs","grant_id":"","title":null},{"funder_name":"Healthy Brains, Healthy Lives","grant_id":"","title":null},{"funder_name":"Canada First Research Excellence Fund","grant_id":"","title":null},{"funder_name":"Healthy Brains, Healthy Lives","grant_id":"","title":null},{"funder_name":"Alain and Sandra Bouchard Foundation","grant_id":"","title":null},{"funder_name":"Canada Research Chairs","grant_id":"","title":null},{"funder_name":"Québec Consortium for Drug Discovery","grant_id":"","title":null},{"funder_name":"Chamandy Foundation","grant_id":"","title":null}],"total_grants":15,"fwci":1.169,"citation_percentile":0.76288247,"influential_citations":0,"citation_trend":[{"year":2025,"count":5},{"year":2026,"count":2}],"oa_status":"hybrid","license":"cc-by-nc","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/biot.202400070","host_type":"journal"},{"url":"https://doi.org/10.1002/biot.202400070","host_type":"HYBRID"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/biot.202400070","host_type":"publisher"},{"url":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/pdf/10.1002/biot.202400070","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/39167558","host_type":"repository"}],"fields_of_study":["3D Printing in Biomedical Research","Pluripotent Stem Cells Research","Planarian Biology and Electrostimulation","Medicine","Engineering","Biology","0301 basic medicine","0303 health sciences","03 medical and health sciences","Organoids","Coculture Techniques","Animals","Cell Movement","Brain","Mesencephalon","Mice","Lab-On-A-Chip Devices","Axons","Microtechnology","Humans","Neurons"],"mesh_terms":["Animals","Axons","Brain","Cell Movement","Humans","Mesencephalon","Neurons","Organoids","Coculture Techniques","Mice","Microtechnology","Lab-On-A-Chip Devices"],"keywords":["Organoid","Midbrain","Cell biology","Biology","Chemistry","Neuroscience","Central nervous system","Co‐culture","Organ‐on‐a‐chip","Brain Organoid","Assembloid","Neurons","Brain","Coculture Techniques","Axons","Organoids","Mice","Cell Movement","Mesencephalon","Lab-On-A-Chip Devices","Animals","Microtechnology","Humans"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-02T16:37:26.581683Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}