{"doi":"10.1002/anse.202100021","title":"<sup>13</sup> C‐Labeled Diethyl Ketoglutarate Derivatives as Hyperpolarized Probes of 2‐Ketoglutarate Dehydrogenase Activity","abstract":"Abstract The TCA cycle is a central metabolic pathway for energy production and biosynthesis. A major control point of metabolic flux through the cycle is the decarboxylation of 2‐ketoglutarate by the TCA cycle enzyme 2‐ketoglutarate dehydrogenase (2‐KGDH). In this project, we developed 13 C labeled 2‐ketoglutarate derivatives to monitor 2‐KGDH activity in vivo. 13 C NMR analysis of liver extracts revealed that uniformly 13 C labeled 2‐ketogutarate, in its cell permeable ester form, was rapidly taken up and hydrolyzed in liver and underwent extensive metabolism to produce labeled glutamate, succinate, lactate and other metabolites. Diethyl [1,2‐ 13 C 2 ]‐2‐ketoglutarate was successfully polarized by dynamic nuclear polarization and within seconds after injection into rats, the probe produced hyperpolarized [ 13 C]bicarbonate in the liver reflecting flux through the TCA cycle. These experiments demonstrate that this tracer offers the possibility of directly monitoring flux through 2‐KGDH in vivo.","journal":"Analysis & Sensing","year":2021,"id":195659,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9589,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":376965,"name":"Eul Hyun Suh","orcid":"0000-0002-3919-678X","position":1,"is_corresponding":false},{"id":240337,"name":"Gaurav Sharma","orcid":"0000-0002-1754-7163","position":2,"is_corresponding":false},{"id":363652,"name":"Jun Chen","orcid":"0000-0002-5814-1653","position":3,"is_corresponding":false},{"id":363653,"name":"Edward P. Hackett","orcid":"0000-0002-7344-6076","position":4,"is_corresponding":false},{"id":767624,"name":"Xiaodong Wen","orcid":null,"position":5,"is_corresponding":false},{"id":240338,"name":"A. Dean Sherry","orcid":"0000-0001-7150-8301","position":6,"is_corresponding":false},{"id":240340,"name":"Chalermchai Khemtong","orcid":"0000-0003-1976-8098","position":7,"is_corresponding":false},{"id":240339,"name":"Craig R. Malloy","orcid":"0000-0001-5077-0642","position":8,"is_corresponding":false},{"id":363655,"name":"Jae Mo Park","orcid":"0000-0002-7404-6971","position":9,"is_corresponding":false},{"id":363654,"name":"Zoltán Kovács","orcid":"0000-0003-0657-3419","position":10,"is_corresponding":false},{"id":373446,"name":"Jaspal Singh","orcid":"0000-0001-5867-2932","position":0,"is_corresponding":true}],"reference_count":32,"raw_metadata":null,"created_at":"2026-07-18T23:50:07.691929Z","pmid":"35669533","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}