{"doi":"10.1002/anie.202309744","title":"Stereoselective Synthesis of Sialyl Lewis<sup>a</sup> Antigen and the Effective Anticancer Activity of Its Bacteriophage Qβ Conjugate as an Anticancer Vaccine","abstract":"Abstract Sialyl Lewis a (sLe a ), also known as cancer antigen 19‐9 (CA19‐9), is a tumor‐associated carbohydrate antigen. The overexpression of sLe a on the surface of a variety of cancer cells makes it an attractive target for anticancer immunotherapy. However, sLe a ‐based anticancer vaccines have been under‐explored. To develop a new vaccine, efficient stereoselective synthesis of sLe a with an amine‐bearing linker was achieved, which was subsequently conjugated with a powerful carrier bacteriophage, Qβ. Mouse immunization with the Qβ‐sLe a conjugate generated strong and long‐lasting anti‐sLe a IgG antibody responses, which were superior to those induced by the corresponding conjugate of sLe a with the benchmark carrier keyhole limpet hemocyanin. Antibodies elicited by Qβ‐sLe a were highly selective toward the sLe a structure, could bind strongly with sLe a ‐expressing cancer cells and human pancreatic cancer tissues, and kill tumor cells through complement‐mediated cytotoxicity. Furthermore, vaccination with Qβ‐sLe a significantly reduced tumor development in a metastatic cancer model in mice, demonstrating tumor protection for the first time by a sLe a ‐based vaccine, thus highlighting the significant potential of sLe a as a promising cancer antigen.","journal":"Angewandte Chemie International Edition","year":2023,"id":341699,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9649,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":490927,"name":"Sherif Ramadan","orcid":"0000-0002-8639-4105","position":1,"is_corresponding":false},{"id":1078241,"name":"Nicholas A. O'Brien","orcid":null,"position":2,"is_corresponding":false},{"id":1077697,"name":"Athar Nakisa","orcid":"0000-0001-7656-9795","position":3,"is_corresponding":false},{"id":308596,"name":"Shuyao Lang","orcid":"0000-0003-1800-7641","position":4,"is_corresponding":false},{"id":225419,"name":"Howard C. Crawford","orcid":"0000-0001-9360-3019","position":5,"is_corresponding":false},{"id":519329,"name":"Jeffrey C. Gildersleeve","orcid":"0000-0002-3744-6423","position":6,"is_corresponding":false},{"id":308597,"name":"Xuefei Huang","orcid":"0000-0002-6468-5526","position":7,"is_corresponding":false},{"id":474163,"name":"Zahra Rashidijahanabad","orcid":null,"position":0,"is_corresponding":true}],"reference_count":89,"raw_metadata":null,"created_at":"2026-07-19T01:11:08.077724Z","pmid":"37781858","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}