{"doi":"10.1002/anie.202309291","title":"Site‐Specific m<sup>6</sup>A Erasing via Conditionally Stabilized CRISPR‐Cas13b Editor","abstract":"Abstract N6‐methyladenosine (m 6 A) on RNAs plays an important role in regulating various biological processes and CRIPSR technology has been employed for programmable m 6 A editing. However, the bulky size of CRISPR protein and constitutively expressed CRISPR/RNA editing enzymes can interfere with the native function of target RNAs and cells. Herein, we reported a conditional m 6 A editing platform (FKBP*‐dCas13b‐ALK) based on a ligand stabilized dCas13 editor. The inducible expression of this m 6 A editing system was achieved by adding or removing the Shield‐1 molecule. We further demonstrated that the targeted recruitment of dCas13b‐m 6 A eraser fusion protein and site‐specific m 6 A erasing were achieved under the control of Shield‐1. Moreover, the release and degradation of dCas13b fusion protein occurred faster than the restoration of m 6 A on the target RNAs after Shield‐1 removal, which provides an ideal opportunity to study the m 6 A function with minimal steric interference from bulky dCas13b fusion protein.","journal":"Angewandte Chemie International Edition","year":2023,"id":344724,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9495,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1083596,"name":"Yufan Wang","orcid":"0009-0000-5306-6749","position":1,"is_corresponding":false},{"id":368791,"name":"Fu‐Sen Liang","orcid":"0000-0002-1793-0824","position":2,"is_corresponding":false},{"id":638330,"name":"Ying Xu","orcid":"0000-0001-7341-8676","position":0,"is_corresponding":true}],"reference_count":46,"raw_metadata":null,"created_at":"2026-07-19T01:11:35.133818Z","pmid":"37713087","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}