{"doi":"10.1002/anie.202300961","title":"Photoredox‐Catalyzed Labeling of Hydroxyindoles with Chemoselectivity (PhotoCLIC) for Site‐Specific Protein Bioconjugation","abstract":"We have developed a novel visible-light-catalyzed bioconjugation reaction, PhotoCLIC, that enables chemoselective attachment of diverse aromatic amine reagents onto a site-specifically installed 5-hydroxytryptophan residue (5HTP) on full-length proteins of varied complexity. The reaction uses catalytic amounts of methylene blue and blue/red light-emitting diodes (455/650 nm) for rapid site-specific protein bioconjugation. Characterization of the PhotoCLIC product reveals a unique structure formed likely through a singlet oxygen-dependent modification of 5HTP. PhotoCLIC has a wide substrate scope and its compatibility with strain-promoted azide-alkyne click reaction, enables site-specific dual-labeling of a target protein.","journal":"Angewandte Chemie International Edition","year":2023,"id":341113,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.953,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":987774,"name":"Conor Loynd","orcid":null,"position":1,"is_corresponding":false},{"id":987775,"name":"Delilah Jewel","orcid":null,"position":2,"is_corresponding":false},{"id":927214,"name":"Sarah E. Canarelli","orcid":null,"position":3,"is_corresponding":false},{"id":976932,"name":"Elise D. Ficaretta","orcid":null,"position":4,"is_corresponding":false},{"id":1077170,"name":"Quan A. Pham","orcid":null,"position":5,"is_corresponding":false},{"id":135937,"name":"Eranthie Weerapana","orcid":"0000-0002-0835-8301","position":6,"is_corresponding":false},{"id":117143,"name":"Abhishek Chatterjee","orcid":"0000-0002-6231-5302","position":7,"is_corresponding":false},{"id":116739,"name":"Soumya Jyoti Singha Roy","orcid":"0000-0002-7999-4591","position":0,"is_corresponding":true}],"reference_count":63,"raw_metadata":null,"created_at":"2026-07-19T01:11:03.393978Z","pmid":"37219923","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}