{"doi":"10.1002/anie.202107829","title":"Programmable Site‐Specific Functionalization of DNA Origami with Polynucleotide Brushes","abstract":"Combining surface-initiated, TdT (terminal deoxynucleotidyl transferase) catalyzed enzymatic polymerization (SI-TcEP) with precisely engineered DNA origami nanostructures (DONs) presents an innovative pathway for the generation of stable, polynucleotide brush-functionalized DNA nanostructures. We demonstrate that SI-TcEP can site-specifically pattern DONs with brushes containing both natural and non-natural nucleotides. The brush functionalization can be precisely controlled in terms of the location of initiation sites on the origami core and the brush height and composition. Coarse-grained simulations predict the conformation of the brush-functionalized DONs that agree well with the experimentally observed morphologies. We find that polynucleotide brush-functionalization increases the nuclease resistance of DONs significantly, and that this stability can be spatially programmed through the site-specific growth of polynucleotide brushes. The ability to site-specifically decorate DONs with brushes of natural and non-natural nucleotides provides access to a large range of functionalized DON architectures that would allow for further supramolecular assembly, and for potential applications in smart nanoscale delivery systems.","journal":"Angewandte Chemie International Edition","year":2021,"id":186170,"datarank":0.4943755299006494,"base_score":3.295836866004329,"endowment":3.295836866004329,"self_citation_contribution":0.4943755299006494,"citation_network_contribution":0.0,"self_endowment_contribution":0.4943755299006494,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":26,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9522,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":743465,"name":"Qinyi Lu","orcid":"0000-0003-1261-9102","position":1,"is_corresponding":false},{"id":743466,"name":"Chao‐Min Huang","orcid":"0000-0002-5304-5013","position":2,"is_corresponding":false},{"id":744165,"name":"Hongji Qian","orcid":null,"position":3,"is_corresponding":false},{"id":743467,"name":"Yunlong Zhang","orcid":"0000-0002-3374-1337","position":4,"is_corresponding":false},{"id":743468,"name":"Sonal Deshpande","orcid":"0000-0003-3055-0065","position":5,"is_corresponding":false},{"id":557018,"name":"Gaurav Arya","orcid":"0000-0002-5615-0521","position":6,"is_corresponding":false},{"id":313823,"name":"Yonggang Ke","orcid":"0000-0003-1673-2153","position":7,"is_corresponding":false},{"id":313943,"name":"Stefan Zauscher","orcid":"0000-0002-2290-7178","position":8,"is_corresponding":false},{"id":743464,"name":"Yunqi Yang","orcid":"0000-0002-6668-2628","position":0,"is_corresponding":true}],"reference_count":47,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:48:42.632147Z","pmid":"34302317","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}