{"doi":"10.1002/anie.202006531","title":"An Integrated Microfluidic Probe for Mass Spectrometry Imaging of Biological Samples**","abstract":"Ambient ionization based on liquid extraction is widely used in mass spectrometry imaging (MSI) of molecules in biological samples. The development of nanospray desorption electrospray ionization (nano-DESI) has enabled the robust imaging of tissue sections with high spatial resolution. However, the fabrication of the nano-DESI probe is challenging, which limits its dissemination to the broader scientific community. Herein, we describe the design and performance of an integrated microfluidic probe (iMFP) for nano-DESI MSI. The glass iMFP, fabricated using photolithography, wet etching, and polishing, shows comparable performance to the capillary-based nano-DESI MSI in terms of stability and sensitivity; a spatial resolution of better than 25 μm was obtained in these first proof-of-principle experiments. The iMFP is easy to operate and align in front of a mass spectrometer, which will facilitate broader use of liquid-extraction-based MSI in biological research, drug discovery, and clinical studies.","journal":"Angewandte Chemie International Edition","year":2020,"id":92941,"datarank":0.5606504427425053,"base_score":3.7376696182833684,"endowment":3.7376696182833684,"self_citation_contribution":0.5606504427425053,"citation_network_contribution":0.0,"self_endowment_contribution":0.5606504427425053,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":41,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9492,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":435860,"name":"Ruichuan Yin","orcid":"0000-0001-8478-7100","position":1,"is_corresponding":false},{"id":435861,"name":"Hang Hu","orcid":"0000-0002-8590-9787","position":2,"is_corresponding":false},{"id":436871,"name":"Yingju Li","orcid":null,"position":3,"is_corresponding":false},{"id":435862,"name":"Xiaofei Sun","orcid":"0000-0001-9601-5423","position":4,"is_corresponding":false},{"id":237670,"name":"Sudhansu K. Dey","orcid":"0000-0001-9159-186X","position":5,"is_corresponding":false},{"id":435863,"name":"Julia Laskin","orcid":"0000-0002-4533-9644","position":6,"is_corresponding":false},{"id":435859,"name":"Xiangtang Li","orcid":"0000-0002-5891-3502","position":0,"is_corresponding":true}],"reference_count":41,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T22:30:31.563852Z","pmid":"32743957","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}