{"doi":"10.1002/anie.201910115","title":"Protein‐Specific, Multicolor and 3D STED Imaging in Cells with DNA‐Labeled Antibodies","abstract":"<jats:title>Abstract</jats:title><jats:p>Photobleaching is a major challenge in fluorescence microscopy, in particular if high excitation light intensities are used. Signal‐to‐noise and spatial resolution may be compromised, which limits the amount of information that can be extracted from an image. Photobleaching can be bypassed by using exchangeable labels, which transiently bind to and dissociate from a target, thereby replenishing the destroyed labels with intact ones from a reservoir. Here, we demonstrate confocal and STED microscopy with short, fluorophore‐labeled oligonucleotides that transiently bind to complementary oligonucleotides attached to protein‐specific antibodies. The constant exchange of fluorophore labels in DNA‐based STED imaging bypasses photobleaching that occurs with covalent labels. We show that this concept is suitable for targeted, two‐color STED imaging of whole cells.</jats:p>","journal":"Angewandte Chemie International Edition","year":2019,"id":594513,"datarank":0.5955437870328184,"base_score":3.970291913552122,"endowment":3.970291913552122,"self_citation_contribution":0.5955437870328184,"citation_network_contribution":0.0,"self_endowment_contribution":0.5955437870328184,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":52,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1521957,"name":"Florian Hurter","orcid":null,"position":1,"is_corresponding":false},{"id":960137,"name":"Mathilda Glaesmann","orcid":null,"position":2,"is_corresponding":false},{"id":267178,"name":"Christos Karathanasis","orcid":"0000-0002-1333-2341","position":3,"is_corresponding":false},{"id":1222980,"name":"Marko Lampe","orcid":"0000-0002-4510-9048","position":4,"is_corresponding":false},{"id":267183,"name":"Mike Heilemann","orcid":"0000-0002-9821-3578","position":5,"is_corresponding":false},{"id":1521955,"name":"Christoph Spahn","orcid":"0000-0001-9886-2263","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Protein-Specific, Multicolor and 3D STED Imaging in Cells with DNA-Labeled Antibodies.","abstract":"Photobleaching is a major challenge in fluorescence microscopy, in particular if high excitation light intensities are used. Signal-to-noise and spatial resolution may be compromised, which limits the amount of information that can be extracted from an image. Photobleaching can be bypassed by using exchangeable labels, which transiently bind to and dissociate from a target, thereby replenishing the destroyed labels with intact ones from a reservoir. Here, we demonstrate confocal and STED microscopy with short, fluorophore-labeled oligonucleotides that transiently bind to complementary oligonucleotides attached to protein-specific antibodies. The constant exchange of fluorophore labels in DNA-based STED imaging bypasses photobleaching that occurs with covalent labels. We show that this concept is suitable for targeted, two-color STED imaging of whole cells.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"31603612","pmcid":"PMC6972974","openalex_id":null,"authors":[],"funders":[{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"HE 6166/17-1","title":null},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"SFB 902","title":null},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"SFB 1177","title":null},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"SFB 807","title":null},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"HE 6166/11-1","title":null},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"161793742/SFB 902","title":"Molecular Principles of RNA-based Regulation"},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"57566863/SFB 807","title":"Transport and Communication Across Biological Membrans"},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"unidentified","title":"unidentified"}],"total_grants":8,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/anie.201910115","host_type":"publisher"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6972974","host_type":"repository"},{"url":"https://nbn-resolving.org/urn:nbn:de:hebis:30:3-638176","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC6972974","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC6972974?pdf=render","host_type":"Europe_PMC"},{"url":"https://doi.org/10.1002/ange.201910115","host_type":""},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ange.201910115","host_type":""},{"url":"https://doi.org/10.1002/anie.201910115","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/31603612","host_type":""},{"url":"http://dx.doi.org/10.1002/anie.201910115","host_type":""},{"url":"https://dx.doi.org/10.1002/anie.201910115","host_type":""},{"url":"https://doi.org/https://doi.org/10.1002/anie.201910115","host_type":""}],"fields_of_study":["0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Hela Cells","Humans","Proteins","DNA","Antibodies","Photobleaching"],"keywords":["Fluorescence","Fluorescent probes","Sted Microscopy","Multicolor Imaging","Dna-paint","570","Photobleaching","ddc:540","Proteins","DNA","540","Communications","Antibodies","Humans","ddc:570","HeLa Cells"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-27T14:47:58.089145Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}