{"doi":"10.1002/ange.202404044","title":"Final Stages in the Biosynthesis of the [FeFe]‐Hydrogenase Active Site","abstract":"Abstract The paper aims to elucidate the final stages in the biosynthesis of the [2Fe] H active site of the [FeFe]‐hydrogenases. The recently hypothesized intermediate [Fe 2 (SCH 2 NH 2 ) 2 (CN) 2 (CO) 4 ] 2− ([ 1 ] 2− ) was prepared by a multistep route from [Fe 2 (S 2 )(CN)(CO) 5 ] − . The following synthetic intermediates were characterized in order: [Fe 2 (SCH 2 NHFmoc) 2 (CNBEt 3 )(CO) 5 ] − , [Fe 2 (SCH 2 NHFmoc) 2 (CN)−(CO) 5 ] − , and [Fe 2 (SCH 2 NHFmoc) 2 (CN) 2 (CO) 4 ] 2− , where Fmoc is fluorenylmethoxycarbonyl). Derivatives of these anions include [K(18‐crown‐6)] + , PPh 4 + and PPN + salts as well as the 13 CD 2 ‐isotopologues. These Fe 2 species exist as a mixture of two isomers attributed to diequatorial (ee) and axial‐equatorial (ae) stereochemistry at sulfur. In vitro experiments demonstrate that [ 1 ] 2− maturates HydA1 in the presence of HydF and a cocktail of reagents. HydA1 can also be maturated using a highly simplified cocktail, omitting HydF and other proteins. This result is consistent with HydA1 participating in the maturation process and refines the roles of HydF.","journal":"Angewandte Chemie","year":2024,"id":461958,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9552,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":334946,"name":"Guodong Rao","orcid":"0000-0001-8043-3436","position":1,"is_corresponding":false},{"id":334945,"name":"R. David Britt","orcid":"0000-0003-0889-8436","position":2,"is_corresponding":false},{"id":225328,"name":"Thomas B. Rauchfuss","orcid":"0000-0003-2547-5128","position":3,"is_corresponding":false},{"id":1113353,"name":"Xin Yu","orcid":"0000-0003-0543-5567","position":0,"is_corresponding":true}],"reference_count":31,"raw_metadata":null,"created_at":"2026-07-19T02:04:16.410510Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}