{"doi":"10.1002/ange.202402353","title":"An Expanded Palette of Fluorescent COS/H<sub>2</sub>S‐Releasing Donors for H<sub>2</sub>S Delivery, Detection, and In Vivo Application","abstract":"Abstract Hydrogen sulfide (H 2 S) is an important reactive sulfur species that is involved in many biological functions, and H 2 S imbalances have been indicated as a potential biomarker for various diseases. Different H 2 S donors have been developed to deliver H 2 S directly to biological systems, but few reports include donors with optical responses that allow for tracking of H 2 S release. Moreover, donor systems that use the same chemistry to deliver H 2 S across a palette of fluorescent responses remain lacking. Here we report five thiol‐activated fluorescence turn‐on COS/H 2 S donors that utilize blue, yellow, orange, red, and near infrared‐emitting dyes functionalized with an H 2 S‐releasing sulfenyl thiocarbonate scaffold. Upon treatment with thiols, each donor provides a fluorescence turn‐on response (3–310‐fold) and high H 2 S release efficiencies (&gt;60 %). Using combined electrode and fluorescence experiments, we directly correlate the measured H 2 S release with the fluorescence response. All donors are biocompatible and release H 2 S in live cell environments. In addition, we demonstrate that the NIR donor allows for imaging H 2 S release in live rats via subcutaneous injection of the donor loaded into an alginate gel, which to the best of our knowledge is the first in vivo tracking of H 2 S release from a fluorogenic donor in non‐transparent organisms.","journal":"Angewandte Chemie","year":2024,"id":479850,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9571,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1071015,"name":"Jonathan Dorogin","orcid":"0000-0003-4694-1415","position":1,"is_corresponding":false},{"id":1212938,"name":"Payton M. Jefferis","orcid":null,"position":2,"is_corresponding":false},{"id":263352,"name":"Marian H. Hettiaratchi","orcid":"0000-0001-8187-4575","position":3,"is_corresponding":false},{"id":325445,"name":"Michael D. Pluth","orcid":"0000-0003-3604-653X","position":4,"is_corresponding":false},{"id":931616,"name":"Kaylin G. Fosnacht","orcid":null,"position":0,"is_corresponding":true}],"reference_count":50,"raw_metadata":null,"created_at":"2026-07-19T02:06:54.284166Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}