{"doi":"10.1002/aic.17440","title":"Antibody screening at reduced <scp>pH</scp> enables preferential selection of potently neutralizing antibodies targeting <scp>SARS‐CoV</scp>‐2","abstract":"Antiviral monoclonal antibody (mAb) discovery enables the development of antibody-based antiviral therapeutics. Traditional antiviral mAb discovery relies on affinity between antibody and a viral antigen to discover potent neutralizing antibodies, but these approaches are inefficient because many high affinity mAbs have no neutralizing activity. We sought to determine whether screening for anti-SARS-CoV-2 mAbs at reduced pH could provide more efficient neutralizing antibody discovery. We mined the antibody response of a convalescent COVID-19 patient at both physiological pH (7.4) and reduced pH (4.5), revealing that SARS-CoV-2 neutralizing antibodies were preferentially enriched in pH 4.5 yeast display sorts. Structural analysis revealed that a potent new antibody called LP5 targets the SARS-CoV-2 N-terminal domain supersite via a unique binding recognition mode. Our data combine with evidence from prior studies to support antibody screening at pH 4.5 to accelerate antiviral neutralizing antibody discovery.","journal":"AIChE Journal","year":2021,"id":205935,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9487,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":527381,"name":"Eswar R. Reddem","orcid":"0000-0003-3054-5506","position":1,"is_corresponding":false},{"id":105619,"name":"Pengfei Wang","orcid":"0000-0003-2454-7652","position":2,"is_corresponding":false},{"id":615367,"name":"Ryan G. Casner","orcid":"0000-0002-2910-0908","position":3,"is_corresponding":false},{"id":105620,"name":"Manoj S. Nair","orcid":"0000-0002-5994-3957","position":4,"is_corresponding":false},{"id":105637,"name":"Yaoxing Huang","orcid":"0000-0001-6270-1644","position":5,"is_corresponding":false},{"id":302417,"name":"Ahmed S. Fahad","orcid":"0000-0002-5088-9333","position":6,"is_corresponding":false},{"id":302420,"name":"Matheus Oliveira de Souza","orcid":"0000-0003-0357-1995","position":7,"is_corresponding":false},{"id":302416,"name":"Bailey B. Banach","orcid":"0000-0003-0208-5856","position":8,"is_corresponding":false},{"id":302418,"name":"Sheila N. López Acevedo","orcid":"0000-0003-2778-4696","position":9,"is_corresponding":false},{"id":302421,"name":"Xiaoli Pan","orcid":"0000-0002-5033-7272","position":10,"is_corresponding":false},{"id":656162,"name":"Rajani Nimrania","orcid":null,"position":11,"is_corresponding":false},{"id":229156,"name":"I‐Ting Teng","orcid":"0000-0002-4704-7100","position":12,"is_corresponding":false},{"id":327783,"name":"Fabiana Bahna","orcid":null,"position":13,"is_corresponding":false},{"id":105631,"name":"Tongqing Zhou","orcid":"0000-0002-3935-4637","position":14,"is_corresponding":false},{"id":229158,"name":"Baoshan Zhang","orcid":"0000-0002-0165-1513","position":15,"is_corresponding":false},{"id":105635,"name":"Michael T. Yin","orcid":"0000-0002-9346-9056","position":16,"is_corresponding":false},{"id":104013,"name":"David D. Ho","orcid":"0000-0003-1627-149X","position":17,"is_corresponding":false},{"id":105633,"name":"Peter D. Kwong","orcid":"0000-0003-3560-232X","position":18,"is_corresponding":false},{"id":105638,"name":"Lawrence Shapiro","orcid":"0000-0001-9943-8819","position":19,"is_corresponding":false},{"id":108827,"name":"Brandon J. DeKosky","orcid":"0000-0001-6406-0836","position":20,"is_corresponding":false},{"id":302419,"name":"Bharat Madan","orcid":"0000-0002-7376-9991","position":0,"is_corresponding":true}],"reference_count":65,"raw_metadata":null,"created_at":"2026-07-18T23:51:37.630221Z","pmid":"34898670","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}