{"doi":"10.1002/advs.202403503","title":"Humoral Immunity Profiling to Pandemic and Bat‐Derived Coronavirus Variants: A Geographical Comparison","abstract":"Dynamic pathogen exposure may impact the immunological response to SARS-CoV-2 (SCV2). One potential explanation for the lack of severe SCV2-related morbidity and mortality in Southeast Asia is prior exposure to related betacoronaviruses. Recent discoveries of SCV2-related betacoronaviruses from horseshoe bats (Rhinolophus sinicus) in Thailand, Laos, and Cambodia suggest the potential for bat-to-human spillover exposures in the region. In this work, serum antibodies to protein constructs from SCV2 and a representative bat coronavirus isolated in Cambodia (RshSTT182) are measured in pre-pandemic Cambodian human sera using ELISA assays. Of 293 Cambodian samples tested (N = 131 with acute malaria, n = 162 with acute undifferentiated febrile illness), 32 (10.9%) are seropositive for SCV2 based on established Spike and receptor-binding domain (RBD) cutoffs. Within SCV2 seropositive samples, 16 (50%) have higher antibody levels to antigens from the representative virus RshSTT182 versus SCV2 antigens; competitive binding ELISA assays demonstrate inhibition of reactivity to SCV2 Spike after pre-incubation with RshSTT182 Spike. Surrogate virus neutralization tests demonstrate that 8/30 (26.7%) SCV2 ELISA positive pre-pandemic Cambodian samples have neutralizing activity against SCV2, while 14/30 (46.7%) have activity against other SCV2-related betacoronaviruses. These data suggest that exposure to related betacoronaviruses may elicit cross-reactive immunity to SCV2 prior to the global pandemic.","journal":"Advanced Science","year":2024,"id":505476,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9584,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":390323,"name":"Andrea Lucia Alfonso","orcid":"0000-0003-1506-531X","position":1,"is_corresponding":false},{"id":517426,"name":"Christina Yek","orcid":"0000-0001-8293-3901","position":2,"is_corresponding":false},{"id":683429,"name":"Zoe Putman","orcid":"0000-0002-8451-6818","position":3,"is_corresponding":false},{"id":267810,"name":"Matthew Drew","orcid":"0000-0002-3954-6671","position":4,"is_corresponding":false},{"id":267809,"name":"Dominic Esposito","orcid":"0000-0002-9987-1687","position":5,"is_corresponding":false},{"id":395417,"name":"Irfan Zaidi","orcid":"0000-0001-9161-2050","position":6,"is_corresponding":false},{"id":552656,"name":"Sophana Chea","orcid":"0000-0003-3187-3691","position":7,"is_corresponding":false},{"id":808617,"name":"Sokna Ly","orcid":null,"position":8,"is_corresponding":false},{"id":808618,"name":"Rathanak Sath","orcid":null,"position":9,"is_corresponding":false},{"id":290868,"name":"Chanthap Lon","orcid":"0000-0003-4901-5003","position":10,"is_corresponding":false},{"id":808212,"name":"Chea Huch","orcid":"0000-0003-4791-9412","position":11,"is_corresponding":false},{"id":510618,"name":"Rithea Leang","orcid":"0000-0003-2645-7618","position":12,"is_corresponding":false},{"id":236329,"name":"Rekol Huy","orcid":"0000-0001-8552-3369","position":13,"is_corresponding":false},{"id":553340,"name":"Sovann Ly","orcid":null,"position":14,"is_corresponding":false},{"id":553342,"name":"Heng Seng","orcid":null,"position":15,"is_corresponding":false},{"id":233415,"name":"Chee Wah Tan","orcid":"0000-0001-9837-1413","position":16,"is_corresponding":false},{"id":233411,"name":"Feng Zhu","orcid":"0000-0002-8131-1219","position":17,"is_corresponding":false},{"id":227144,"name":"Lin‐Fa Wang","orcid":"0000-0003-2752-0535","position":18,"is_corresponding":false},{"id":271926,"name":"Fabiano Oliveira","orcid":"0000-0002-7924-8038","position":19,"is_corresponding":false},{"id":238245,"name":"Kaitlyn Sadtler","orcid":"0000-0003-2587-8330","position":20,"is_corresponding":false},{"id":344856,"name":"Jessica E. 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