{"doi":"10.1002/advs.202206872","title":"CRISPR‐Enhanced Hydrogel Microparticles for Multiplexed Detection of Nucleic Acids","abstract":"CRISPR/Cas systems offer a powerful sensing mechanism to transduce sequence-specific information into amplified analytical signals. However, performing multiplexed CRISPR/Cas assays remains challenging and often requires complex approaches for multiplexed assays. Here, a hydrogel-based CRISPR/Cas12 system termed CLAMP (Cas-Loaded Annotated Micro-Particles) is described. The approach compartmentalizes the CRISPR/Cas reaction in spatially-encoded hydrogel microparticles (HMPs). Each HMP is identifiable by its face code and becomes fluorescent when target DNA is present. The assay is further streamlined by capturing HMPs inside a microfluidic device; the captured particles are then automatically recognized by a machine-learning algorithm. The CLAMP assay is fast, highly sensitive (attomolar detection limits with preamplification), and capable of multiplexing in a single-pot assay. As a proof-of-concept clinical application, CLAMP is applied to detect nucleic acid targets of human papillomavirus in cervical brushing samples.","journal":"Advanced Science","year":2023,"id":318104,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":68,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9486,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":241882,"name":"Chang Yeol Lee","orcid":"0000-0001-5382-8635","position":1,"is_corresponding":false},{"id":1024640,"name":"Su-Jin Lee","orcid":"0000-0002-0477-7568","position":2,"is_corresponding":false},{"id":275926,"name":"Hyunho Kim","orcid":"0000-0003-3072-6526","position":3,"is_corresponding":false},{"id":873608,"name":"Amy Ly","orcid":"0000-0002-4888-5026","position":4,"is_corresponding":false},{"id":789066,"name":"Cesar M. Castro","orcid":"0000-0002-1159-5658","position":5,"is_corresponding":false},{"id":241886,"name":"Jinwoo Cheon","orcid":"0000-0001-8948-5929","position":6,"is_corresponding":false},{"id":241885,"name":"Jae‐Hyun Lee","orcid":"0000-0002-9236-157X","position":7,"is_corresponding":false},{"id":241884,"name":"Hakho Lee","orcid":"0000-0002-0087-0909","position":8,"is_corresponding":false},{"id":1024639,"name":"Yoon Ho Roh","orcid":"0000-0003-4341-5372","position":0,"is_corresponding":true}],"reference_count":47,"raw_metadata":null,"created_at":"2026-07-19T01:06:55.884740Z","pmid":"36725305","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}