{"doi":"10.1002/admi.201700702","title":"bFGF and Poly‐RGD Cooperatively Establish Biointerface for Stem Cell Adhesion, Proliferation, and Differentiation","abstract":"<jats:title>Abstract</jats:title><jats:p>Biointerface design is widely used to functionalize biomaterials with controllable physicochemical properties. Functionalized biointerface provides a versatile platform to connect biological entities and nonbiogenic materials. Existing nanofabrication approaches to create such a nanostructured biointerface involve in low stability of the functionalized nanolayer and simple functionalities that limit its applicability. Here, a stable nanolayered synthetic polypeptide (poly[LA‐<jats:italic>co</jats:italic>‐(Glc‐alt‐Lys)] and modified with arginine‐glycine‐aspartic acid, PRGD)/basic fibroblast growth factor (bFGF) biointerface is created via structural matching, charge interaction, and hydrogen bonding. The cooperative effect of the PRGD/bFGF biointerface shows multiple functionalities in promoting stem cell adhesion by 33% increase in cell adhesion to poly(<jats:sc>d,l</jats:sc>‐lactic acid) substrate as compared to experiments on bare substrate as a control. Moreover, the biointerface enhances proliferation by 40% in cell density, potential differentiation by 62%, and gene expression by 40 and 80% respectively as compared to the control samples. The fabricated biointerface may have applications in nerve regeneration, tissue repair, and stem cell‐based therapy.</jats:p>","journal":"Advanced Materials Interfaces","year":2018,"id":665858,"datarank":0.40620753016533157,"base_score":2.70805020110221,"endowment":2.70805020110221,"self_citation_contribution":0.40620753016533157,"citation_network_contribution":0.0,"self_endowment_contribution":0.40620753016533157,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":459723,"name":"Yong Wang","orcid":"0000-0003-2507-8333","position":1,"is_corresponding":false},{"id":1738744,"name":"Yi‐Xia Yin","orcid":null,"position":2,"is_corresponding":false},{"id":1738745,"name":"Rui‐Peng Wei","orcid":null,"position":3,"is_corresponding":false},{"id":1738747,"name":"Guo‐Liang Ying","orcid":null,"position":4,"is_corresponding":false},{"id":1738748,"name":"Bin‐Bin Li","orcid":null,"position":5,"is_corresponding":false},{"id":353286,"name":"Tong Qiu","orcid":"0000-0003-4499-437X","position":6,"is_corresponding":false},{"id":1738750,"name":"Patrick van Rijn","orcid":null,"position":7,"is_corresponding":false},{"id":1464673,"name":"Ge Tian","orcid":"0000-0003-1306-652X","position":8,"is_corresponding":false},{"id":1738751,"name":"Qiong‐Jiao Yan","orcid":null,"position":9,"is_corresponding":false},{"id":1738752,"name":"Hong‐Lian Dai","orcid":null,"position":10,"is_corresponding":false},{"id":275958,"name":"Henk J. Busscher","orcid":"0000-0002-3644-5533","position":11,"is_corresponding":false},{"id":1738753,"name":"Shi‐Pu Li","orcid":null,"position":12,"is_corresponding":false},{"id":653833,"name":"Ali K. Yetisen","orcid":"0000-0003-0896-267X","position":13,"is_corresponding":false},{"id":1738755,"name":"Xiao‐Yu Yang","orcid":"0000-0003-3454-3604","position":14,"is_corresponding":false},{"id":1380681,"name":"Nan Jiang","orcid":"0000-0002-8849-7976","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"bFGF and Poly‐RGD Cooperatively Establish Biointerface for Stem Cell Adhesion, Proliferation, and Differentiation","abstract":"<jats:title>Abstract</jats:title><jats:p>Biointerface design is widely used to functionalize biomaterials with controllable physicochemical properties. Functionalized biointerface provides a versatile platform to connect biological entities and nonbiogenic materials. Existing nanofabrication approaches to create such a nanostructured biointerface involve in low stability of the functionalized nanolayer and simple functionalities that limit its applicability. Here, a stable nanolayered synthetic polypeptide (poly[LA‐<jats:italic>co</jats:italic>‐(Glc‐alt‐Lys)] and modified with arginine‐glycine‐aspartic acid, PRGD)/basic fibroblast growth factor (bFGF) biointerface is created via structural matching, charge interaction, and hydrogen bonding. The cooperative effect of the PRGD/bFGF biointerface shows multiple functionalities in promoting stem cell adhesion by 33% increase in cell adhesion to poly(<jats:sc>d,l</jats:sc>‐lactic acid) substrate as compared to experiments on bare substrate as a control. Moreover, the biointerface enhances proliferation by 40% in cell density, potential differentiation by 62%, and gene expression by 40 and 80% respectively as compared to the control samples. The fabricated biointerface may have applications in nerve regeneration, tissue repair, and stem cell‐based therapy.</jats:p>","is_dataset_classified":null,"base_score":2.70805020110221,"endowment":2.70805020110221,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19162232","pmcid":null,"openalex_id":"https://openalex.org/W2789353566","authors":[],"funders":[],"total_grants":0,"fwci":0.8306,"citation_percentile":0.67993863,"influential_citations":0,"citation_trend":[{"year":2018,"count":4},{"year":2019,"count":2},{"year":2020,"count":1},{"year":2021,"count":2},{"year":2022,"count":1},{"year":2023,"count":2},{"year":2024,"count":2}],"oa_status":"bronze","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/admi.201700702","host_type":"journal"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/admi.201700702","host_type":"publisher"},{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fadmi.201700702","host_type":"publisher"},{"url":"https://advanced.onlinelibrary.wiley.com/doi/pdf/10.1002/admi.201700702","host_type":"publisher"},{"url":"https://doi.org/10.1002/admi.201700702","host_type":"journal"},{"url":"https://research.rug.nl/en/publications/9d15666d-2d67-494a-b536-5247ad6da806","host_type":"repository"},{"url":"https://hdl.handle.net/11370/9d15666d-2d67-494a-b536-5247ad6da806","host_type":"repository"}],"fields_of_study":["Graphene and Nanomaterials Applications","Bone Tissue Engineering Materials","Electrospun Nanofibers in Biomedical Applications"],"mesh_terms":[],"keywords":["Biointerface","Materials science","Adhesion","Nanotechnology","Cell adhesion","Substrate (aquarium)","Tissue engineering","Biomedical engineering","Biology","Composite material","Engineering"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-13T12:28:28.663738Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}