{"doi":"10.1002/adhm.202304386","title":"Deterministic Single‐Cell Encapsulation in PEG Norbornene Microgels for Promoting Anti‐Inflammatory Response and Therapeutic Delivery of Mesenchymal Stromal Cells","abstract":"Tissue engineering at single-cell resolution has enhanced therapeutic efficacy. Droplet microfluidics offers a powerful platform that allows deterministic single-cell encapsulation into aqueous droplets, yet the direct encapsulation of cells into microgels remains challenging. Here, the design of a microfluidic device that is capable of single-cell encapsulation within polyethylene glycol norbornene (PEGNB) hydrogels on-chip is reported. Cells are first ordered in media within a straight microchannel via inertial focusing, followed by the introduction of PEGNB solution from two separate, converging channels. Droplets are thoroughly mixed by passage through a serpentine channel, and microgels are formed by photo-photopolymerization. This platform uniquely enables both single-cell encapsulation and excellent cell viability post-photo-polymerization. More than 90% of singly encapsulated mesenchymal stromal cells (MSCs) remain alive for 7 days. Notably, singly encapsulated MSCs have elevated expression levels in genes that code anti-inflammatory cytokines, for example, IL-10 and TGF-β, thus enhancing the secretion of proteins of interest. Following injection into a mouse model with induced inflammation, singly encapsulated MSCs show a strong retention rate in vivo, reduce overall inflammation, and mitigate liver damage. These translational results indicate that deterministic single-cell encapsulation could find use in a broad spectrum of tissue engineering applications.","journal":"Advanced Healthcare Materials","year":2024,"id":430958,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":17,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9425,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1234781,"name":"Yuanzhuo Chen","orcid":null,"position":1,"is_corresponding":false},{"id":493686,"name":"Kun Jiang","orcid":"0000-0003-1525-1227","position":2,"is_corresponding":false},{"id":308371,"name":"Ke Ma","orcid":"0000-0003-1257-6034","position":3,"is_corresponding":false},{"id":1234338,"name":"Edward D. Ramsey","orcid":"0000-0002-8217-0905","position":4,"is_corresponding":false},{"id":491637,"name":"John Oakey","orcid":"0000-0003-1023-1964","position":5,"is_corresponding":false},{"id":560593,"name":"Mingming Sun","orcid":"0000-0002-3117-7066","position":6,"is_corresponding":false},{"id":493683,"name":"Zhongliang Jiang","orcid":"0000-0003-3146-8282","position":7,"is_corresponding":false},{"id":1234780,"name":"Hangjun Si","orcid":null,"position":0,"is_corresponding":true}],"reference_count":54,"raw_metadata":null,"created_at":"2026-07-19T01:59:20.861374Z","pmid":"38373601","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}