{"doi":"10.1002/adhm.202200804","title":"The Modular µSiM: A Mass Produced, Rapidly Assembled, and Reconfigurable Platform for the Study of Barrier Tissue Models In Vitro","abstract":"Advanced in vitro tissue chip models can reduce and replace animal experimentation and may eventually support \"on-chip\" clinical trials. To realize this potential, however, tissue chip platforms must be both mass-produced and reconfigurable to allow for customized design. To address these unmet needs, an extension of the µSiM (microdevice featuring a silicon-nitride membrane) platform is introduced. The modular µSiM (m-µSiM) uses mass-produced components to enable rapid assembly and reconfiguration by laboratories without knowledge of microfabrication. The utility of the m-µSiM is demonstrated by establishing an hiPSC-derived blood-brain barrier (BBB) in bioengineering and nonengineering, brain barriers focused laboratories. In situ and sampling-based assays of small molecule diffusion are developed and validated as a measure of barrier function. BBB properties show excellent interlaboratory agreement and match expectations from literature, validating the m-µSiM as a platform for barrier models and demonstrating successful dissemination of components and protocols. The ability to quickly reconfigure the m-µSiM for coculture and immune cell transmigration studies through addition of accessories and/or quick exchange of components is then demonstrated. Because the development of modified components and accessories is easily achieved, custom designs of the m-µSiM shall be accessible to any laboratory desiring a barrier-style tissue chip platform.","journal":"Advanced Healthcare Materials","year":2022,"id":243613,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":33,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9534,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":678663,"name":"Pelin Kasap","orcid":"0000-0003-3423-7359","position":1,"is_corresponding":false},{"id":385527,"name":"S. Danial Ahmad","orcid":"0000-0003-3956-0139","position":2,"is_corresponding":false},{"id":457137,"name":"Shiuan‐Haur Su","orcid":"0000-0002-8956-3533","position":3,"is_corresponding":false},{"id":876027,"name":"Kaihua Chen","orcid":"0000-0003-1042-3050","position":4,"is_corresponding":false},{"id":686474,"name":"Mehran Mansouri","orcid":"0000-0002-2949-1857","position":5,"is_corresponding":false},{"id":876706,"name":"Natalie Ramesh","orcid":null,"position":6,"is_corresponding":false},{"id":279346,"name":"Hideaki Nishihara","orcid":"0000-0002-2990-835X","position":7,"is_corresponding":false},{"id":876028,"name":"Yury Belyaev","orcid":"0000-0001-8318-2951","position":8,"is_corresponding":false},{"id":686475,"name":"Vinay V. Abhyankar","orcid":"0000-0002-8462-3920","position":9,"is_corresponding":false},{"id":876029,"name":"Stefano Begolo","orcid":"0000-0002-1361-8200","position":10,"is_corresponding":false},{"id":372182,"name":"Benjamin H. Singer","orcid":"0000-0002-4721-6920","position":11,"is_corresponding":false},{"id":876030,"name":"Kevin F. Webb","orcid":"0000-0002-0858-304X","position":12,"is_corresponding":false},{"id":457144,"name":"Katsuo Kurabayashi","orcid":"0000-0002-9613-3590","position":13,"is_corresponding":false},{"id":261505,"name":"Jonathan Flax","orcid":null,"position":14,"is_corresponding":false},{"id":471333,"name":"Richard E. Waugh","orcid":"0000-0003-2084-5740","position":15,"is_corresponding":false},{"id":279353,"name":"Britta Engelhardt","orcid":"0000-0003-3059-9846","position":16,"is_corresponding":false},{"id":385528,"name":"James L. McGrath","orcid":"0000-0003-2017-8335","position":17,"is_corresponding":false},{"id":352981,"name":"Molly C. McCloskey","orcid":"0000-0002-5237-8422","position":0,"is_corresponding":true}],"reference_count":79,"raw_metadata":null,"created_at":"2026-07-19T00:23:20.901576Z","pmid":"35899801","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}