{"doi":"10.1002/adhm.202200206","title":"A Localized Materials‐Based Strategy to Non‐Virally Deliver Chondroitinase ABC mRNA Improves Hindlimb Function in a Rat Spinal Cord Injury Model","abstract":"Spinal cord injury often results in devastating consequences for those afflicted, with very few therapeutic options. A central element of spinal cord injuries is astrogliosis, which forms a glial scar that inhibits neuronal regeneration post-injury. Chondroitinase ABC (ChABC) is an enzyme capable of degrading chondroitin sulfate proteoglycan (CSPG), the predominant extracellular matrix component of the glial scar. However, poor protein stability remains a challenge in its therapeutic use. Messenger RNA (mRNA) delivery is an emerging gene therapy technology for in vivo production of difficult-to-produce therapeutic proteins. Here, mineral-coated microparticles as an efficient, non-viral mRNA delivery vehicles to produce exogenous ChABC in situ within a spinal cord lesion are used. ChABC production reduces the deposition of CSPGs in an in vitro model of astrogliosis, and direct injection of these microparticles within a glial scar forces local overexpression of ChABC and improves recovery of motor function seven weeks post-injury.","journal":"Advanced Healthcare Materials","year":2022,"id":250652,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":26,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9552,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":615444,"name":"Daniel J. Hellenbrand","orcid":"0000-0003-4730-5752","position":1,"is_corresponding":false},{"id":893486,"name":"Kaitlyn Reichl","orcid":null,"position":2,"is_corresponding":false},{"id":476451,"name":"Jennifer M. Umhoefer","orcid":"0000-0002-8563-9641","position":3,"is_corresponding":false},{"id":892865,"name":"Mallory Filipp","orcid":"0000-0003-2957-3753","position":4,"is_corresponding":false},{"id":892866,"name":"Joshua A. Choe","orcid":"0000-0003-4757-8849","position":5,"is_corresponding":false},{"id":615446,"name":"Amgad S. Hanna","orcid":"0000-0002-5062-5952","position":6,"is_corresponding":false},{"id":395184,"name":"William L. Murphy","orcid":"0000-0001-5577-7739","position":7,"is_corresponding":false},{"id":307121,"name":"Andrew Khalil","orcid":"0000-0003-1526-6656","position":0,"is_corresponding":true}],"reference_count":79,"raw_metadata":null,"created_at":"2026-07-19T00:24:32.960657Z","pmid":"35882512","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}