{"doi":"10.1002/adhm.202102123","title":"Template‐Enabled Biofabrication of Thick 3D Tissues with Patterned Perfusable Macrochannels","abstract":"Interconnected pathways in 3D bioartificial organs are essential to retaining cell activity in thick functional 3D tissues. 3D bioprinting methods have been widely explored in biofabrication of functionally patterned tissues; however, these methods are costly and confined to thin tissue layers due to poor control of low-viscosity bioinks. Here, cell-laden hydrogels that could be precisely patterned via water-soluble gelatin templates are constructed by economical extrusion 3D printed plastic templates. Tortuous co-continuous plastic networks, designed based on triply periodic minimal surfaces (TPMS), serve as a sacrificial pattern to shape the secondary sacrificial gelatin templates. These templates are eventually used to form cell-encapsulated gelatin methacryloyl (GelMA) hydrogel scaffolds patterned with the complex interconnected pathways. The proposed fabrication process is compatible with photo-crosslinkable hydrogels wherein prepolymer casting enables incorporation of high cell populations with high viability. The cell-laden hydrogel constructs are characterized by robust mechanical behavior. In vivo studies demonstrate a superior cell ingrowth into the highly permeable constructs compared to the bulk hydrogels. Perfusable complex interconnected networks within cell-encapsulated hydrogels may assist in engineering thick and functional tissue constructs through the permeable internal channels for efficient cellular activities in vivo.","journal":"Advanced Healthcare Materials","year":2021,"id":170500,"datarank":0.9391365848484294,"base_score":3.367295829986474,"endowment":3.367295829986474,"self_citation_contribution":0.5050943744979712,"citation_network_contribution":0.43404221035045826,"self_endowment_contribution":0.5050943744979712,"citer_contribution":0.43404221035045826,"corpus_percentile":null,"corpus_rank":null,"citation_count":28,"citer_count":23,"citers_with_citation_signal":19,"citers_with_endowment":19,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9464,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":245312,"name":"Hossein Montazerian","orcid":"0000-0001-6972-2667","position":1,"is_corresponding":false},{"id":622743,"name":"Masoud Zhianmanesh","orcid":"0000-0001-9645-1276","position":2,"is_corresponding":false},{"id":402111,"name":"Reza Abbasgholizadeh","orcid":"0000-0003-0904-6489","position":3,"is_corresponding":false},{"id":241782,"name":"Reihaneh Haghniaz","orcid":"0000-0003-0033-891X","position":4,"is_corresponding":false},{"id":258846,"name":"Avijit Baidya","orcid":"0000-0001-5215-2856","position":5,"is_corresponding":false},{"id":703361,"name":"Homeyra Pourmohammadali","orcid":"0000-0001-8545-4981","position":6,"is_corresponding":false},{"id":230208,"name":"Nasim Annabi","orcid":"0000-0003-1879-1202","position":7,"is_corresponding":false},{"id":255732,"name":"Paul S. Weiss","orcid":"0000-0001-5527-6248","position":8,"is_corresponding":false},{"id":245318,"name":"Ehsan Toyserkani","orcid":"0000-0002-6716-9500","position":9,"is_corresponding":false},{"id":230339,"name":"Ali Khademhosseini","orcid":"0000-0002-2692-1524","position":10,"is_corresponding":false},{"id":245310,"name":"Elham Davoodi","orcid":"0000-0001-8578-9431","position":0,"is_corresponding":true}],"reference_count":57,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:46:24.040760Z","pmid":"34967148","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}