{"doi":"10.1002/adhm.202001094","title":"Tissue Engineered Vascular Graft Recipient Interleukin 10 Status Is Critical for Preventing Thrombosis","abstract":"Tissue engineered vascular grafts (TEVGs) are a promising technology, but are hindered by occlusion. Seeding with bone-marrow derived mononuclear cells (BM-MNCs) mitigates occlusion, yet the precise mechanism remains unclear. Seeded cells disappear quickly and potentially mediate an anti-inflammatory effect through paracrine signaling. Here, a series of reciprocal genetic TEVG implantations plus recombinant protein treatment is reported to investigate what role interleukin-10, an anti-inflammatory cytokine, plays from both host and seeded cells. TEVGs seeded with BM-MNCs from wild-type and IL-10 KO mice, plus unseeded grafts, are implanted into wild-type and IL-10 KO mice. Wild-type mice with unseeded grafts also receive recombinant IL-10. Serial ultrasound evaluates occlusion and TEVGs are harvested at 14 d for immunohistochemical analysis. TEVGs in IL-10 KO mice have significantly higher occlusion incidence compared to wild-type mice attributed to acute (<3 d) thrombosis. Cell seeding rescues TEVGs in IL-10 KO mice comparable to wild-type patency. IL-10 from the host and seeded cells do not significantly influence graft inflammation and macrophage phenotype, yet IL-10 treatment shows interesting biologic effects including decreasing cell proliferation and increasing M2 macrophage polarization. IL-10 from the host is critical for preventing TEVG thrombosis and seeded BM-MNCs exert a significant anti-thrombotic effect in IL-10 KO mice.","journal":"Advanced Healthcare Materials","year":2020,"id":88526,"datarank":0.46803883335281976,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"self_citation_contribution":0.3596842909197557,"citation_network_contribution":0.10835454243306408,"self_endowment_contribution":0.3596842909197557,"citer_contribution":0.10835454243306408,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":5,"citers_with_citation_signal":5,"citers_with_endowment":5,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9584,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":448936,"name":"Jenny C. Barker","orcid":"0000-0002-1517-5124","position":1,"is_corresponding":false},{"id":268872,"name":"Jacob C. Zbinden","orcid":"0000-0002-0262-383X","position":2,"is_corresponding":false},{"id":450085,"name":"Brevan M. Santantonio","orcid":null,"position":3,"is_corresponding":false},{"id":384516,"name":"Yu‐Chun Chang","orcid":null,"position":4,"is_corresponding":false},{"id":268867,"name":"Cameron A. Best","orcid":"0000-0001-7426-0137","position":5,"is_corresponding":false},{"id":268881,"name":"James W. Reinhardt","orcid":"0000-0001-8406-8421","position":6,"is_corresponding":false},{"id":268876,"name":"Kevin M. Blum","orcid":"0000-0002-8346-629X","position":7,"is_corresponding":false},{"id":448937,"name":"Tai Yi","orcid":"0000-0003-0464-1043","position":8,"is_corresponding":false},{"id":268894,"name":"Christopher K. Breuer","orcid":"0000-0003-0987-3898","position":9,"is_corresponding":false},{"id":268873,"name":"Gabriel Mirhaidari","orcid":"0000-0003-0665-7268","position":0,"is_corresponding":true}],"reference_count":53,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T22:01:22.387926Z","pmid":"33073543","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}