{"doi":"10.1002/acn3.52237","title":"In vivo evidence for cell body loss in cortical lesions in people with multiple sclerosis","abstract":"OBJECTIVE: To quantify alterations in soma and neurite density imaging measures within and surrounding cortical lesions in people with multiple sclerosis using in vivo high-gradient diffusion MRI. METHODS: In this cross-sectional study, 41 people with multiple sclerosis and 34 age- and sex-matched healthy controls underwent 3 T high-gradient diffusion MRI. Cortical lesions were segmented on artificial intelligence-enabled double inversion recovery images. \"Inner\" and \"outer\" perilesional layers were segmented as two expanding shells of 2 mm surrounding a cortical lesion. Intracellular, intra-neurite, and extracellular signal fractions and apparent soma radius were estimated in (peri)lesional and normal-appearing cortex. RESULTS: Cortical lesions were present in all people with multiple sclerosis with a median count of 8 [IQR 5-18] and total volume of 0.16 [0.09-0.46 mL]. People with multiple sclerosis (mean 0.27 ± 0.03) showed lower normalized cortical volumes compared to healthy controls (0.30 ± 0.02). Compared to healthy controls (mean 0.58 ± 0.028), normal-appearing cortex in multiple sclerosis (0.57 ± 0.034) showed lower intra-cellular signal fraction. Cortical lesions (0.49 ± 0.089) exhibited lower intra-cellular signal fractions compared to perilesional (\"inner\": 0.55 ± 0.049, \"outer\": 0.55 ± 0.039) and normal-appearing cortex, demonstrating a gradation of change. The soma radius varied significantly across cortices, becoming smaller when moving outward from cortical lesions (cortical lesions: 10.38 ± 0.209 μm, \"inner\" layer: 10.19 ± 0.140 μm, \"outer\" layer: 10.07 ± 0.149 μm, normal-appearing cortex: 9.99 ± 0.127 μm). INTERPRETATION: Cortical cell body loss in multiple sclerosis is most pronounced in cortical lesions and also present in normal-appearing cortex. Gradients of diffusion microstructural alterations moving outward from cortical lesions toward normal-appearing cortex highlight the potential of high-gradient diffusion MRI to identify both focal and diffuse cortical pathology.","journal":"Annals of Clinical and Translational Neurology","year":2024,"id":448071,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.85,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1050538,"name":"Hansol Lee","orcid":"0000-0003-2112-1197","position":1,"is_corresponding":false},{"id":1266721,"name":"Samantha Noteboom","orcid":"0000-0003-4796-8636","position":2,"is_corresponding":false},{"id":759860,"name":"Florence L. Chiang","orcid":"0000-0002-3405-5590","position":3,"is_corresponding":false},{"id":1266722,"name":"Martijn D. Steenwijk","orcid":"0000-0002-8346-9044","position":4,"is_corresponding":false},{"id":931876,"name":"Menno M. Schoonheim","orcid":"0000-0002-2504-6959","position":5,"is_corresponding":false},{"id":297461,"name":"Eric C. Klawiter","orcid":"0000-0002-3429-2633","position":6,"is_corresponding":false},{"id":263270,"name":"Susie Y. Huang","orcid":"0000-0003-2950-7254","position":7,"is_corresponding":false},{"id":1050537,"name":"Eva A. Krijnen","orcid":"0000-0002-3628-4127","position":0,"is_corresponding":true}],"reference_count":50,"raw_metadata":null,"created_at":"2026-07-19T02:02:08.018689Z","pmid":"39673156","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}