{"doi":"10.1002/2211-5463.13008","title":"The rapid endocytic uptake of fetuin‐A by adherent tumor cells is mediated by Toll‐like receptor 4 (TLR4)","abstract":"Fetuin-A is a serum glycoprotein synthesized and secreted into blood by the liver and whose main physiological function is the inhibition of ectopic calcification. However, a number of studies have demonstrated that it is a multifunctional protein. For example, endocytic uptake of fetuin-A by tumor cells resulting in rapid cellular adhesion and spreading has been reported. The precise uptake mechanism, however, has been elusive. The present studies were done to determine whether Toll-like receptor-4 (TLR4), which has been previously shown to be a receptor for fetuin-A and is commonly expressed in immune cells, could take part in the rapid uptake (< 3 min) of fetuin-A by tumor cells. Rapid uptake of fetuin-A was inhibited by the specific TLR4 inhibitor CLI-095 and also attenuated in TLR4 knockdown prostate tumor cells. Inhibition of TLR4 by CLI-095 also attenuated the rapid adhesion of tumor cells as well as invasion through a bed of Matrigel. The data suggest mechanisms by which TLR4 modulates the adhesion and growth of tumor cells.","journal":"FEBS Open Bio","year":2020,"id":108285,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9381,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":379600,"name":"Gladys N. Nangami","orcid":null,"position":1,"is_corresponding":false},{"id":519103,"name":"Tanu Rana","orcid":"0000-0002-4428-2754","position":2,"is_corresponding":false},{"id":519104,"name":"Adam G. Evans","orcid":"0000-0001-8535-7583","position":3,"is_corresponding":false},{"id":338899,"name":"Stephen D. Williams","orcid":"0000-0003-1960-8158","position":4,"is_corresponding":false},{"id":519926,"name":"Dylan Crowell","orcid":null,"position":5,"is_corresponding":false},{"id":250501,"name":"Anil Shanker","orcid":"0000-0001-6372-3669","position":6,"is_corresponding":false},{"id":338903,"name":"Amos M. Sakwe","orcid":"0000-0002-2283-794X","position":7,"is_corresponding":false},{"id":338901,"name":"Josiah Ochieng","orcid":"0000-0003-4551-5174","position":8,"is_corresponding":false},{"id":422102,"name":"Portia Thomas","orcid":"0000-0002-1374-5015","position":0,"is_corresponding":true}],"reference_count":34,"raw_metadata":null,"created_at":"2026-07-18T23:12:43.081337Z","pmid":"33073533","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}