{"doi":"10.1002/2211-5463.12570","title":"Recurrent activations of transient receptor potential vanilloid‐1 and vanilloid‐4 promote cellular proliferation and migration in esophageal squamous cell carcinoma cells","abstract":"Some members of the transient receptor potential vanilloid (TRPV) subfamily of cation channels are thermosensitive. Earlier studies have revealed the distribution and functions of these thermo‐TRPVs (TRPV1–4) in various organs, but their expression and function in the human esophagus are not fully understood. Here, we probed for the expression of the thermo‐TRPVs in one nontumor human esophageal squamous cell line and two esophageal squamous cell carcinoma (ESCC) cell lines. TRPV1, TRPV2, and TRPV4 proteins were found to be upregulated in ESCC cells, while TRPV3 was not detectable in any of these cell lines. Subsequently, channel function was evaluated via monitoring of Ca2+ transients by Ca2+ imaging and nonselective cation channel currents were recorded by whole‐cell patch clamp. We found that TRPV4 was activated by heat at 28 °C–35 °C, whereas TRPV1 and TRPV2 were activated by higher, noxious temperatures (44 °C and 53 °C, respectively). Furthermore, TRPV1 was activated by capsaicin (EC50 = 20.32 μm), and this effect was antagonized by AMG9810; TRPV2 was activated by a newly developed cannabinoid compound, O1821, and inhibited by tranilast. In addition, TRPV4 was activated by hypotonic solutions (220 m Osm), and this effect was abolished by ruthenium red. The effects of TRPV1 and TRPV4 on ESCC were also explored. Our data, for the first time, showed that the overactivation of TRPV1 and TRPV4 promoted the proliferation and/or migration of ESCC cells. In summary, TRPV1, TRPV2, and TRPV4 were functionally expressed in human esophageal squamous cells, and thermo‐TRPVs might play an important role in the development of ESCC.","journal":"FEBS Open Bio","year":2019,"id":26771,"datarank":1.4573942444734893,"base_score":3.8712010109078907,"endowment":3.8712010109078907,"self_citation_contribution":0.5806801516361837,"citation_network_contribution":0.8767140928373056,"self_endowment_contribution":0.5806801516361837,"citer_contribution":0.8767140928373056,"corpus_percentile":null,"corpus_rank":null,"citation_count":47,"citer_count":43,"citers_with_citation_signal":35,"citers_with_endowment":35,"datacite_reuse_total":2,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":110276,"name":"Fei Wang","orcid":"0000-0002-1244-3680","position":1,"is_corresponding":false},{"id":115692,"name":"Yuchen Yang","orcid":null,"position":2,"is_corresponding":false},{"id":154133,"name":"Wenbo Ma","orcid":null,"position":3,"is_corresponding":false},{"id":154134,"name":"Zuoxian Lin","orcid":null,"position":4,"is_corresponding":false},{"id":154135,"name":"Na Cheng","orcid":null,"position":5,"is_corresponding":false},{"id":154136,"name":"Yan Long","orcid":null,"position":6,"is_corresponding":false},{"id":154137,"name":"Sihao Deng","orcid":null,"position":7,"is_corresponding":false},{"id":120792,"name":"Zhiyuan Li","orcid":null,"position":8,"is_corresponding":false},{"id":154132,"name":"Rongqi Huang","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.8712010109078907,"endowment":3.8712010109078907,"datacite_reuse_total":2,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"30761248","pmcid":null,"openalex_id":"https://openalex.org/W2903842813","authors":[],"funders":[{"funder_name":"National Natural Science Foundation of China","grant_id":"31671211; 81503170","title":null},{"funder_name":"Natural Science Foundation of Guangdong Province","grant_id":"2014B090901036; 2014A020215029; 408150736021; 2016","title":null}],"total_grants":2,"fwci":2.1915,"citation_percentile":0.86653314,"influential_citations":1,"citation_trend":[{"year":2019,"count":2},{"year":2020,"count":9},{"year":2021,"count":5},{"year":2022,"count":12},{"year":2023,"count":6},{"year":2024,"count":4},{"year":2025,"count":6},{"year":2026,"count":3}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://febs.onlinelibrary.wiley.com/doi/pdfdirect/10.1002/2211-5463.12570","host_type":"journal"},{"url":"https://febs.onlinelibrary.wiley.com/doi/pdfdirect/10.1002/2211-5463.12570","host_type":"GOLD"},{"url":"https://febs.onlinelibrary.wiley.com/doi/pdfdirect/10.1002/2211-5463.12570","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/2211-5463.12570","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/full-xml/10.1002/2211-5463.12570","host_type":"publisher"},{"url":"https://febs.onlinelibrary.wiley.com/doi/pdf/10.1002/2211-5463.12570","host_type":"publisher"},{"url":"https://doi.org/10.1002/2211-5463.12570","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/30761248","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6356177","host_type":"repository"}],"fields_of_study":["Ion Channels and Receptors","Respiratory and Cough-Related Research","Chemistry","Medicine","Biology","Cell Movement","Cell Proliferation","Cells, Cultured","Esophageal Neoplasms","Esophageal Squamous Cell Carcinoma","Humans","TRPV Cation Channels"],"mesh_terms":["Esophageal Squamous Cell Carcinoma","Cell Movement","Cells, Cultured","Esophageal Neoplasms","Humans","Cell Proliferation","TRPV Cation Channels"],"keywords":["Transient receptor potential channel","TRPV1","Esophageal squamous cell carcinoma","Cancer research","Capsaicin","Cell growth","Receptor","Chemistry","Basal cell","Carcinoma","Medicine","Internal medicine","Biochemistry","Ca2+ imaging","TRPV","cellular migration","cellular proliferation"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[{"doi":"10.6084/m9.figshare.26657224.v1","title":"Additional file 1 of Capsaicin orchestrates metastasis in gastric cancer via modulating expression of TRPV1 channels and driving gut microbiota disorder","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.26657224","title":"Additional file 1 of Capsaicin orchestrates metastasis in gastric cancer via modulating expression of TRPV1 channels and driving gut microbiota disorder","publisher":"figshare","resource_type":"JournalArticle"}],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-08T15:39:41.052548Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}