{"doi":"10.1002/1878-0261.13644","title":"Levels of circulating tumor\n                    <scp>DNA</scp>\n                    correlate with tumor volume in gastro‐intestinal stromal tumors: an exploratory long‐term follow‐up study","abstract":"<jats:p>\n                    Patients with gastro‐intestinal stromal tumors (GISTs) undergoing tyrosine kinase inhibitor therapy are monitored with regular computed tomography (CT) scans, exposing patients to cumulative radiation. This exploratory study aimed to evaluate circulating tumor DNA (ctDNA) testing to monitor treatment response and compare changes in ctDNA levels with RECIST 1.1 and total tumor volume measurements. Between 2014 and 2021, six patients with KIT proto‐oncogene, receptor tyrosine kinase (\n                    <jats:italic>KIT</jats:italic>\n                    ) exon‐11‐mutated GIST from whom long‐term plasma samples were collected prospectively were included in the study. ctDNA levels of relevant plasma samples were determined using the KIT exon 11 digital droplet PCR drop‐off assay. Tumor volume measurements were performed using a semi‐automated approach. In total, 94 of 130 clinically relevant ctDNA samples were analyzed. Upon successful treatment response, ctDNA became undetectable in all patients. At progressive disease, ctDNA was detectable in five out of six patients. Higher levels of ctDNA correlated with larger tumor volumes. Undetectable ctDNA at the time of progressive disease on imaging was consistent with lower tumor volumes compared to those with detectable ctDNA. In summary, ctDNA levels seem to correlate with total tumor volume at the time of progressive disease. Our exploratory study shows promise for including ctDNA testing in treatment follow‐up.\n                  </jats:p>","journal":"Molecular Oncology","year":2024,"id":632217,"datarank":0.26876392038420827,"base_score":1.791759469228055,"endowment":1.791759469228055,"self_citation_contribution":0.26876392038420827,"citation_network_contribution":0.0,"self_endowment_contribution":0.26876392038420827,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1638706,"name":"Charlotte M. S. C. Haag","orcid":null,"position":1,"is_corresponding":false},{"id":1638707,"name":"Naomi Rifaela","orcid":null,"position":2,"is_corresponding":false},{"id":1638708,"name":"Gerrieke Beukema","orcid":null,"position":3,"is_corresponding":false},{"id":1638709,"name":"Ron H. J. Mathijssen","orcid":null,"position":4,"is_corresponding":false},{"id":91856,"name":"Neeltje Steeghs","orcid":"0000-0003-2989-2279","position":5,"is_corresponding":false},{"id":260049,"name":"Hans Gelderblom","orcid":"0000-0001-9270-8636","position":6,"is_corresponding":false},{"id":1638710,"name":"Ingrid M. E. Desar","orcid":null,"position":7,"is_corresponding":false},{"id":914054,"name":"Arjen Cleven","orcid":null,"position":8,"is_corresponding":false},{"id":1631915,"name":"Arja ter Elst","orcid":null,"position":9,"is_corresponding":false},{"id":1638711,"name":"Ed Schuuring","orcid":"0000-0003-3655-143X","position":10,"is_corresponding":false},{"id":1638712,"name":"Anna K. L. Reyners","orcid":null,"position":11,"is_corresponding":false},{"id":1638705,"name":"Roos F. Bleckman","orcid":"0000-0001-6701-6208","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Levels of circulating tumor\n                    <scp>DNA</scp>\n                    correlate with tumor volume in gastro‐intestinal stromal tumors: an exploratory long‐term follow‐up study","abstract":"<jats:p>\n                    Patients with gastro‐intestinal stromal tumors (GISTs) undergoing tyrosine kinase inhibitor therapy are monitored with regular computed tomography (CT) scans, exposing patients to cumulative radiation. This exploratory study aimed to evaluate circulating tumor DNA (ctDNA) testing to monitor treatment response and compare changes in ctDNA levels with RECIST 1.1 and total tumor volume measurements. Between 2014 and 2021, six patients with KIT proto‐oncogene, receptor tyrosine kinase (\n                    <jats:italic>KIT</jats:italic>\n                    ) exon‐11‐mutated GIST from whom long‐term plasma samples were collected prospectively were included in the study. ctDNA levels of relevant plasma samples were determined using the KIT exon 11 digital droplet PCR drop‐off assay. Tumor volume measurements were performed using a semi‐automated approach. In total, 94 of 130 clinically relevant ctDNA samples were analyzed. Upon successful treatment response, ctDNA became undetectable in all patients. At progressive disease, ctDNA was detectable in five out of six patients. Higher levels of ctDNA correlated with larger tumor volumes. Undetectable ctDNA at the time of progressive disease on imaging was consistent with lower tumor volumes compared to those with detectable ctDNA. In summary, ctDNA levels seem to correlate with total tumor volume at the time of progressive disease. Our exploratory study shows promise for including ctDNA testing in treatment follow‐up.\n                  </jats:p>","is_dataset_classified":null,"base_score":1.791759469228055,"endowment":1.791759469228055,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"38790141","pmcid":"PMC11547224","openalex_id":"https://openalex.org/W4399019805","authors":[],"funders":[{"funder_name":"KWF Kankerbestrijding","grant_id":"RUG 2013‐635","title":null},{"funder_name":"KWF Kankerbestrijding","grant_id":"RUG 2013-635","title":null}],"total_grants":2,"fwci":1.7417,"citation_percentile":0.82958314,"influential_citations":0,"citation_trend":[{"year":2025,"count":2},{"year":2026,"count":3}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/1878-0261.13644","host_type":"journal"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/1878-0261.13644","host_type":"publisher"},{"url":"https://febs.onlinelibrary.wiley.com/doi/pdf/10.1002/1878-0261.13644","host_type":"publisher"},{"url":"https://doi.org/10.1002/1878-0261.13644","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/38790141","host_type":"repository"},{"url":"https://research.rug.nl/en/publications/4546db4e-bfa1-4a70-b081-d3d12cbc3d2c","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11547224","host_type":"repository"},{"url":"https://pure.eur.nl/en/publications/91dec17d-be9e-49e7-84c4-d0502911e33a","host_type":"repository"},{"url":"https://hdl.handle.net/2066/313757","host_type":"repository"},{"url":"https://hdl.handle.net/1887/4247401","host_type":"repository"},{"url":"https://doaj.org/article/24846d9179084dad86b3bdbabda1dee8","host_type":"repository"},{"url":"https://research.rug.nl/files/1180601023/Molecular_Oncology_-_2024_-_Bleckman_-_Levels_of_circulating_tumor_DNA_correlate_with_tumor_volume_in_gastro_intestinal.pdf","host_type":"repository"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11547224/pdf/MOL2-18-2658.pdf","host_type":"repository"},{"url":"https://pure.eur.nl/ws/files/173389952/Molecular_Oncology_-_2024_-_Bleckman_-_Levels_of_circulating_tumor_DNA_correlate_with_tumor_volume_in_gastro_intestinal.pdf","host_type":"repository"},{"url":"https://repository.ubn.ru.nl//bitstream/handle/2066/313757/313757.pdf","host_type":"repository"},{"url":"https://scholarlypublications.universiteitleiden.nl/access/item%3A4247402/view","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC11547224","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC11547224?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Gastrointestinal Tumor Research and Treatment","Cancer Genomics and Diagnostics","Sarcoma Diagnosis and Treatment"],"mesh_terms":["Circulating Tumor DNA","Proto-Oncogene Mas","Aged","Female","Follow-Up Studies","Gastrointestinal Neoplasms","Humans","Male","Middle Aged","Gastrointestinal Stromal Tumors","Tumor Burden"],"keywords":["Circulating tumor DNA","Stromal tumor","Medicine","Digital polymerase chain reaction","Response Evaluation Criteria in Solid Tumors","GiST","Progressive disease","Pathology","Cancer","Internal medicine","Cancer research","Oncology","Stromal cell","Biology","Disease","Polymerase chain reaction","Treatment","Tumor Volume","Follow‐up","Gastro‐intestinal Stromal Tumor"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"nct"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T09:12:34.805062Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}