{"doi":"10.1002/1873-3468.14348","title":"Oxidation of the Mycobacterium tuberculosis key virulence factor protein tyrosine phosphatase A (MptpA) reduces its phosphatase activity","abstract":"The Mycobacterium tuberculosis tyrosine‐specific phosphatase MptpA and its cognate kinase PtkA are prospective targets for anti‐tuberculosis drugs as they interact with the host defense response within the macrophages. Although both are structurally well‐characterized, the functional mechanism regulating their activity remains poorly understood. Here, we investigate the effect of post‐translational oxidation in regulating the function of MptpA. Treatment of MptpA with H2O2/NaHCO3, mimicking cellular oxidative stress conditions, leads to oxidation of the catalytic cysteine (C11) and to a conformational rearrangement of the phosphorylation loop (D‐loop) by repositioning the conserved tyrosine 128 (Y128) and generating a temporarily inactive preclosed state of the phosphatase. Thus, the catalytic cysteine in the P‐loop acts as a redox switch and regulates the phosphatase activity of MptpA.","journal":"FEBS Letters","year":2022,"id":681294,"datarank":0.24141568686511508,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.0,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":247901,"name":"Sridhar Sreeramulu","orcid":"0000-0003-4509-4568","position":1,"is_corresponding":false},{"id":1780044,"name":"Hendrik R. A. Jonker","orcid":null,"position":2,"is_corresponding":false},{"id":247894,"name":"Christian Richter","orcid":"0000-0002-5420-2826","position":3,"is_corresponding":false},{"id":247900,"name":"Harald Schwalbe","orcid":"0000-0001-5693-7909","position":4,"is_corresponding":false},{"id":900320,"name":"Anna Niesteruk","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Oxidation of the Mycobacterium tuberculosis key virulence factor protein tyrosine phosphatase A (MptpA) reduces its phosphatase activity","abstract":"The Mycobacterium tuberculosis tyrosine‐specific phosphatase MptpA and its cognate kinase PtkA are prospective targets for anti‐tuberculosis drugs as they interact with the host defense response within the macrophages. Although both are structurally well‐characterized, the functional mechanism regulating their activity remains poorly understood. Here, we investigate the effect of post‐translational oxidation in regulating the function of MptpA. Treatment of MptpA with H2O2/NaHCO3, mimicking cellular oxidative stress conditions, leads to oxidation of the catalytic cysteine (C11) and to a conformational rearrangement of the phosphorylation loop (D‐loop) by repositioning the conserved tyrosine 128 (Y128) and generating a temporarily inactive preclosed state of the phosphatase. Thus, the catalytic cysteine in the P‐loop acts as a redox switch and regulates the phosphatase activity of MptpA.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"35397176","pmcid":null,"openalex_id":null,"authors":[],"funders":[{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"FOR2509","title":null},{"funder_name":"Hessisches Ministerium für Wissenschaft und Kunst","grant_id":"BMRZ","title":null},{"funder_name":"FP7 Research infrastructures","grant_id":"iNEXT‐Discovery","title":null},{"funder_name":"FP7 Research infrastructures","grant_id":"871037","title":null},{"funder_name":"Deutschen Konsortium für Translationale Krebsforschung","grant_id":"","title":null}],"total_grants":5,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://doi.org/10.1002/1873-3468.14348","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/1873-3468.14348","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/full-xml/10.1002/1873-3468.14348","host_type":"publisher"},{"url":"https://febs.onlinelibrary.wiley.com/doi/pdf/10.1002/1873-3468.14348","host_type":"publisher"},{"url":"https://nbn-resolving.org/urn:nbn:de:hebis:30:3-706087","host_type":"repository"}],"fields_of_study":[],"mesh_terms":["Mycobacterium tuberculosis","Cysteine","Tyrosine","Bacterial Proteins","Virulence Factors","Prospective Studies","Oxidation-Reduction","Protein Tyrosine Phosphatases"],"keywords":["Mycobacterium tuberculosis","Reactive oxygen species","Protein tyrosine phosphatase","Protein oxidation","Nuclear magnetic resonance spectroscopy","Cysteine-redox Regulation"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T17:27:17.963049Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}