{"doi":"10.1002/1873-3468.12844","title":"Dynamics and function of<scp>ERM</scp>proteins during cytokinesis in human cells","abstract":"<jats:p>The molecular mechanism that governs cytoskeleton–membrane interaction during animal cytokinesis remains elusive. Here, we investigated the dynamics and functions of<jats:styled-content style=\"fixed-case\">ERM</jats:styled-content>(Ezrin/Radixin/Moesin) proteins during cytokinesis in human cultured cells. We found that ezrin is recruited to the cleavage furrow through its membrane‐associated domain in a cholesterol‐dependent but largely Rho‐independent manner. While<jats:styled-content style=\"fixed-case\">ERM</jats:styled-content>s are dispensable for furrow ingression, they play a pivotal role in contractile activity of the polar cortex. Notably, when anillin and supervillin are codepleted,<jats:styled-content style=\"fixed-case\">ERM</jats:styled-content>s increasingly accumulate at the cleavage furrow and substantially contribute to the furrow ingression. These results reveal a supportive role of<jats:styled-content style=\"fixed-case\">ERM</jats:styled-content>s in cortical activities during cytokinesis, and also provide insight into the selective mechanism that preferentially associates cytokinesis‐relevant proteins with the division site.</jats:p>","journal":"FEBS Letters","year":2017,"id":46785,"datarank":0.9980559938127105,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"self_citation_contribution":0.47032413238937254,"citation_network_contribution":0.527731861423338,"self_endowment_contribution":0.47032413238937254,"citer_contribution":0.527731861423338,"corpus_percentile":null,"corpus_rank":null,"citation_count":22,"citer_count":18,"citers_with_citation_signal":17,"citers_with_endowment":17,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":202392,"name":"Tomoko Kamasaki","orcid":null,"position":1,"is_corresponding":false},{"id":216411,"name":"Kohei Otomo","orcid":null,"position":2,"is_corresponding":false},{"id":216412,"name":"Tomomi Nemoto","orcid":null,"position":3,"is_corresponding":false},{"id":202391,"name":"Ryota Uehara","orcid":null,"position":4,"is_corresponding":false},{"id":202393,"name":"Shota Hiruma","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Dynamics and function of<scp>ERM</scp>proteins during cytokinesis in human cells","abstract":"<jats:p>The molecular mechanism that governs cytoskeleton–membrane interaction during animal cytokinesis remains elusive. Here, we investigated the dynamics and functions of<jats:styled-content style=\"fixed-case\">ERM</jats:styled-content>(Ezrin/Radixin/Moesin) proteins during cytokinesis in human cultured cells. We found that ezrin is recruited to the cleavage furrow through its membrane‐associated domain in a cholesterol‐dependent but largely Rho‐independent manner. While<jats:styled-content style=\"fixed-case\">ERM</jats:styled-content>s are dispensable for furrow ingression, they play a pivotal role in contractile activity of the polar cortex. Notably, when anillin and supervillin are codepleted,<jats:styled-content style=\"fixed-case\">ERM</jats:styled-content>s increasingly accumulate at the cleavage furrow and substantially contribute to the furrow ingression. These results reveal a supportive role of<jats:styled-content style=\"fixed-case\">ERM</jats:styled-content>s in cortical activities during cytokinesis, and also provide insight into the selective mechanism that preferentially associates cytokinesis‐relevant proteins with the division site.</jats:p>","is_dataset_classified":null,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"28889652","pmcid":null,"openalex_id":"https://openalex.org/W2752740214","authors":[],"funders":[{"funder_name":"Takeda Science Foundation","grant_id":"","title":null},{"funder_name":"Akiyama Life Science Foundation","grant_id":"","title":null},{"funder_name":"Heiwa Nakajima Foundation","grant_id":"","title":null},{"funder_name":"Inamori Foundation","grant_id":"","title":null},{"funder_name":"Suhara Memorial Foundation","grant_id":"","title":null}],"total_grants":5,"fwci":0.9921,"citation_percentile":0.7357177,"influential_citations":0,"citation_trend":[{"year":2019,"count":4},{"year":2020,"count":4},{"year":2021,"count":5},{"year":2022,"count":2},{"year":2023,"count":3},{"year":2024,"count":2},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"bronze","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/1873-3468.12844","host_type":"journal"},{"url":"https://febs.onlinelibrary.wiley.com/doi/pdfdirect/10.1002/1873-3468.12844","host_type":"BRONZE"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/1873-3468.12844","host_type":"publisher"},{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2F1873-3468.12844","host_type":"publisher"},{"url":"https://febs.onlinelibrary.wiley.com/doi/pdf/10.1002/1873-3468.12844","host_type":"publisher"},{"url":"https://doi.org/10.1002/1873-3468.12844","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/28889652","host_type":"repository"},{"url":"http://hdl.handle.net/2115/71760","host_type":"repository"}],"fields_of_study":["Microtubule and mitosis dynamics","Cellular transport and secretion","Cellular Mechanics and Interactions","Medicine","Chemistry","Biology","Actin Cytoskeleton","Cell Line, Transformed","Cell Membrane","Cytokinesis","Cytoskeletal Proteins","Gene Expression Regulation","Genes, Reporter","Green Fluorescent Proteins","HeLa Cells","Humans","Luciferases","Membrane Proteins","Microfilament Proteins","Molecular Dynamics Simulation","Signal Transduction","rhoA GTP-Binding Protein","Ezrin"],"mesh_terms":["Ezrin","Cell Line, Transformed","Cell Membrane","Cytoskeletal Proteins","Gene Expression Regulation","HeLa Cells","Humans","Luciferases","Membrane Proteins","Microfilament Proteins","Actin Cytoskeleton","Signal Transduction","Genes, Reporter","rhoA GTP-Binding Protein","Cytokinesis","Green Fluorescent Proteins","Molecular Dynamics Simulation","Hela Cells"],"keywords":["Cytokinesis","Cleavage furrow","Cell biology","Ingression","Ezrin","Septin","Moesin","RHOA","Radixin","Midbody","Cell cortex","Biology","Cytoskeleton","Cell division","Chemistry","Cell","Signal transduction","Biochemistry","Ezrin/radixin/moesin"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-14T03:04:33.472809Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}