{"doi":"10.1002/0471142735.im0804s00","title":"One‐Dimensional Gel Electrophoresis of Proteins","abstract":"<jats:title>Abstract</jats:title><jats:p>This unit provides several methods for one‐dimensional gel electrophoresis of proteins. The basic protocol is the standard method used for gel electrophoresis under denaturing conditions. The first alternate protocol provides conditions used for nondenaturing gel electrophoresis. Gradient gels are described and resolve a much wider molecular‐weight range of proteins on a single gel. Minigels, both single‐concentration and gradient, are also described.</jats:p>","journal":"Current Protocols in Immunology","year":1991,"id":25230,"datarank":1.9844796624751764,"base_score":2.995732273553991,"endowment":2.995732273553991,"self_citation_contribution":0.4493598410330987,"citation_network_contribution":1.5351198214420776,"self_endowment_contribution":0.4493598410330987,"citer_contribution":1.5351198214420776,"corpus_percentile":null,"corpus_rank":null,"citation_count":19,"citer_count":19,"citers_with_citation_signal":18,"citers_with_endowment":18,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":14446,"name":"John A. Smith","orcid":"0000-0001-8837-4928","position":1,"is_corresponding":false},{"id":144597,"name":"Sean R. Gallagher","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.995732273553991,"endowment":2.995732273553991,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"18998881","pmcid":null,"openalex_id":"https://openalex.org/W1755728098","authors":[],"funders":[],"total_grants":0,"fwci":0.5837,"citation_percentile":0.54479604,"influential_citations":3,"citation_trend":[{"year":2012,"count":1},{"year":2018,"count":1}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2F0471142735.im0804s00","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/full-xml/10.1002/0471142735.im0804s00","host_type":"publisher"},{"url":"https://currentprotocols.onlinelibrary.wiley.com/doi/full/10.1002/0471142735.im0804s00","host_type":"publisher"},{"url":"https://doi.org/10.1002/0471142735.im0804s00","host_type":"journal"}],"fields_of_study":["Advanced Proteomics Techniques and Applications","Mass Spectrometry Techniques and Applications","Enzyme Structure and Function","Chemistry","Biology"],"mesh_terms":[],"keywords":["Two-dimensional gel electrophoresis","Gel electrophoresis of proteins","Gel electrophoresis","Temperature gradient gel electrophoresis","Electrophoresis","Molecular-weight size marker","Chromatography","Free-flow electrophoresis","Chemistry","Pulsed-field gel electrophoresis","Polyacrylamide gel electrophoresis","Gel electrophoresis of nucleic acids","Biochemistry","Proteomics","Enzyme","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-08T01:13:49.226490Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}